???(???????)?????
|
|
???(???????)????? ??
- ???
- 167-172 °C(lit.)
- ?? ?
- 219-220 °C10 mm Hg(lit.)
- ??
- 1,353 g/cm3
- ???
- 0.0267 hPa (20 °C)
- ???
- 1.4170 (estimate)
- ???
- 219-220°C/10mm
- ?? ??
- 20-25°C
- ???
- H2O: 4 M at 20 °C, ??, ??
- ??? ??
- ?? ??
- ??
- ???
- pH ??
- 7 - 9
- ??????(pH)
- 10.5-12.0(4 m in water, 25 °C)
- ?? ?? (pKa)
- 8.1(at 25℃)
- ???
- 550g/L(25℃)
- ?? ??(λmax)
- λ: 260 nm Amax: 0.10
λ: 280 nm Amax: 0.08
- ??
- Hygroscopic
- Merck
- 14,9772
- BRN
- 741883
- ???
- ????. ??, ?? ???? ???? ????. ????? ??????.
- InChIKey
- LENZDBCJOHFCAS-UHFFFAOYSA-N
- LogP
- -2.515 (est)
- CAS ??????
- 77-86-1(CAS DataBase Reference)
??
- ?? ? ?? ??
- ?? ? ???? ?? (GHS)
| ??? ?? | Xi | ||
|---|---|---|---|
| ?? ???? ?? | 36/37/38 | ||
| ????? | 26-36-37/39-24/25-23 | ||
| WGK ?? | 2 | ||
| RTECS ?? | TY2900000 | ||
| F ?????? | 3 | ||
| TSCA | Yes | ||
| ?? ?? | IRRITANT | ||
| HS ?? | 29221980 | ||
| ?? ?? ??? | 77-86-1(Hazardous Substances Data) | ||
| ?? | LD50 orally in Rabbit: > 5000 mg/kg LD50 dermal Rat > 5000 mg/kg | ||
| ???? ?? | KE-01403 |
???(???????)????? C??? ??, ??, ??
??
???? ???? ????? ??? ?? ??? ??.??? ???? ???? TAE? TBE? ?? ?? ??? ??? ??? ?? ?? ??? ?? ???. ?? ?? ?? ??(Primary Standard)??? ??, ?? ??? ???? ??? ??? ?? ???? ?? ????. ???? ?? TAE ?? TBE ???? ???? ?????? ?????, TAE, TBE ???? ???? ? ????? ??? ? ??? ???? ????? ????.??
??? (??? ?? ??) ??? ?? , ?? ~?? ??? tham ? ???? ??? ?? tae ? tbe ???? ?? ??? ??? ????? ?? ????. ???? 8.06? pka? ??? ???? ??? ????? ?? ?????. ???? ???? ??? (ph 7.0-9.0)? ???? ?? ?? ph ??? ?? ??? ??? ?? ?????. ???? ?? ??? ??? ???????????? ???? ?? ? ? ????? ???????.??
???? ??? : ?? ?? ?? ??? - hcl ph? 7.0 ~ 9.2???. ?? ??? ??? : ?? ???? ?? ??? ?? ????. ?? ??? : ??? ?? ???? ????? ?? ? ???? ?? ?????. ?? ?? ?? ? ??, ?? ???, ?? ??, ??? ??? ??, ??, ???, ??? ?? ? ???? ???. ?? ? ??? : ??? ( ?? ?? ? )? ?? ??? ???? ??? ?? ??? ?? ? ?? ?? ? ??. ?? ??? : ???? ?? ???? ?????. ??? ?? ??? ????? ??? ? ?? ??.??? ??
White, crystalline solid. PH (0.1M aqueous solution) 10.6, corrosive to copper, brass, aluminum. Solubility in water 80 g/100 cc (20C). Combustible.??
Tris base has been used:- In the preparation of TAE buffer for TAE agarose gel used to run PCR products by gel electrophoresis
- As a component of sample buffer for SDS-PAGE
- In the preparation of IEC (ion-exchange chromatography) buffer
?? ??
Tris(hydroxymethyl)aminomethane is prepared industrially by the exhaustive condensation of nitromethane with formaldehyde under basic conditions (i.e. repeated Henry reactions) to produce the intermediate (HOCH2)3CNO2, which is subsequently hydrogenated to give the final product.?? ??
Tris(hydroxymethyl)aminomethane is mainly required for preparation of buffers at physiological range of 7.3 to 7.5. The prepared buffers are compatible with biological fluids. It is of importance to laboratories as a standard pH solution.Tris(hydroxymethyl)aminomethane has been used as a buffer solution for lactate dehydrogenase assay, in situ hybridization procedure and protein extraction from cells.
Tris(hydroxymethyl)aminomethane is used as a buffer in biochemistry and molecular biology laboratories. It is used as a primary standard to standardize acid solutions for chemical analysis. It finds application in cell membranes to increase permeability. As an alternative to sodium bicarbonate, it used in the treatment of metabolic acidosis. It acts as a precursor for the preparation of polymers, oxazolones and oxazolidines.
?? ??
Tris(hydroxymethyl)aminomethane is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.Purification Methods
Tris(hydroxymethyl)aminomethane can ordinarily be obtained in highly pure form suitable for use as an acidimetric standard. If only impure material is available, it should be crystallised from 20% EtOH, aqueous MeOH (m 171.1o) or isopropanol (m 172-173o). Dry it in a vacuum desiccator over P2O5 or CaCl2. Alternatively, it is dissolved in twice its weight of water at 55-60o, filtered, concentrated to half its volume and poured slowly, with stirring, into about twice its volume of EtOH. The crystals which separate on cooling to 3-4o are filtered off, washed with a little MeOH, air dried by suction, then finally ground and dried in a vacuum desiccator over P2O5. It has also been recrystallised from water, MeOH or aqueous MeOH, and vacuum dried at 80o for 2 days. [Beilstein 4 H 303, 4 III 857, 4 IV 1903.]???(???????)????? ?? ?? ? ???
???
?? ??
???(???????)????? ?? ??
???( 1127)?? ??
| ??? | ?? | ??? | ?? | ?? ? | ?? |
|---|---|---|---|---|---|
| Jinan Qinmu Fine Chemical Co.,Ltd. | +8618660799346 |
sales@qinmuchem.com | China | 1009 | 58 |
| Beijing Risun Science and Technology Limited | +86-13311342578 +86-19801193650 |
xykj@risun.com | China | 13 | 58 |
| Hefei TNJ Chemical Industry Co.,Ltd. | +86-0551-65418684 +8618949823763 |
sales@tnjchem.com | China | 25356 | 58 |
| Zouping mingyuan import & export trading co., ltd | +86-0543-2240078 +8618364991597 |
myzhao793@163.com | China | 994 | 58 |
| Henan Allgreen Chemical Co.,LTD | +86-37155567971 +86-13633837469 |
info@allgreenchem.com | China | 5985 | 58 |
| Aladdin Scientific | +1-+1(833)-552-7181 |
sales@aladdinsci.com | United States | 57505 | 58 |
| Wuhan Fortuna Chemical Co., Ltd | +86-027-59207850 |
info@fortunachem.com | China | 5975 | 58 |
| Hebei Chuanghai Biotechnology Co,.LTD | +86-13131129325 |
sales1@chuanghaibio.com | China | 5889 | 58 |
| Sinoway Industrial co., ltd. | 0592-5800732; +8613806035118 |
xie@china-sinoway.com | China | 988 | 58 |
| Hebei Weibang Biotechnology Co., Ltd | +8617732866630 |
bess@weibangbio.com | China | 18151 | 58 |