ChemicalBook >?? ???? >???-AM
???-AM
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???-AM ??
- ???
- >250℃
- ?? ?
- 982.7±65.0 °C(Predicted)
- ??
- 1.49±0.1 g/cm3(Predicted)
- ???
- n
20/D 1.479
- ???
- 85 °C
- ?? ??
- -20°C
- ???
- DMSO: ??? 10mg/mL, ??, ??~?? ???
- ?? ?? (pKa)
- 2.66±0.50(Predicted)
- ??? ??
- ??
- ??
- ???
- ?? ??(λmax)
- < 300 nm
- ??? ??? ??
- Calcium indicators; zinc indicators; cytotoxicity assays; apoptosis assays; viability assays; labile iron pool assays; chemotaxis probes; cell adhesion probes; mitochondrial probes; P-glycoprotein probes;multi-drug resistance probes;treating atherosclerosis,cancer;ischemic disease
??
| ??? ?? | Xi | ||
|---|---|---|---|
| ?? ???? ?? | 36/37/38 | ||
| ????? | 26-36 | ||
| ????(UN No.) | NA 1993 / PGIII | ||
| WGK ?? | 3 | ||
| F ?????? | 10-21 |
???-AM C??? ??, ??, ??
??? ??
A colorless to slightly yellow solid. Soluble in dimethyl sulfoxide.??
Calcein-AM appears to best satisfy the criteria for assaying cell adhesion and to have the least effect on cell viability and cell functions.?? ??
Liposomes contain a self-quenching fluorescent dye called calcein. It is a fluorescent dye with excitation and emission wavelengths of 495/515 nm, respectively. It self-quenches at concentrations above 70mM and is commonly used as an indicator of lipid vesicle leakage. The leakage rate is calculated on the basis of the fluorescence increment as the entrapped calcein is leaked out of the liposomal compartment. It is used as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium.Biochem/physiol Actions
Fluorescent cell permeable derivative of calcein.?? ??
[1] JAMES HYNES . Fluorescence-Based Cell Viability Screening Assays Using Water-Soluble Oxygen Probes[J]. SLAS Discovery, 2003, 8 3: Pages 264-272. DOI:10.1177/1087057103008003004.[2] SABNIS R W. Handbook of Biological Dyes and Stains: Synthesis and Industrial Applications[C]. 2010: 0. DOI:10.1002/9780470586242.
[3] Polli, J.W., Wring, S.A., Humphreys, J.E., et al. Rational use of in vitro P-glycoprotein assays in drug discovery. J. Pharmacol. Exp. Ther. 299(2), 620-628 (2001).
[4] Tiberghien, F., and Loor, F. Ranking of P-glycoprotein substates and inhibitors by a calcein-AM fluorometry screening assay. Anticancer Drugs 7(5), 568-578 (1996).
[5] Szakács, G., Jakab, K., Antal, F., et al. Diagnostics of multidrug resistance in cancer. Pathol.Oncol.Res. 4(4), 251-257 (2016).
[6] X M WANG. A new microcellular cytotoxicity test based on calcein AM release.[J]. Human Immunology, 1993, 37 4: 264-270. DOI:10.1016/0198-8859(93)90510-8.
[7] ZSOLT HOLLó . Calcein accumulation as a fluorometric functional assay of the multidrug transporter[J]. Biochimica et biophysica acta. Biomembranes, 1994, 1191 2: Pages 384-388. DOI:10.1016/0005-2736(94)90190-2.
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???( 148)?? ??
| ??? | ?? | ??? | ?? | ?? ? | ?? |
|---|---|---|---|---|---|
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???-AM ?? ??:
?? ????????? ???? ????????? ??????? ?2? ????? ??(2,2,6,6-?????-3,5-???????) ??????? ?? ???(II) ????????? ??????
Tosylmethyl isocyanide
DICHLORO(ETHYLENEDIAMINE)PLATINUM(II)
SALCOMINE
TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III)
Ethyl isocyanoacetate
PHENYLSELENOL
N-BUTYLISOCYANIDE
TERT-BUTYL ISOCYANIDE
Tris(2,4-pentanedionato)chroMiuM(III)
1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE
Benzyl isocyanide
METHYL ISOCYANOACETATE