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2'',7''-???????????

2'',7''-???????????
2'',7''-??????????? ??? ???
?? ??:
76-54-0
???:
2'',7''-???????????
???(??):
2'',7''-???????????;2',7'-???????????
???:
2',7'-Dichlorofluorescein
???(??):
2,7-DICHLOROFLUORESCEIN;DICHLOROFLUORESCEIN;FLUORESCEIN 27;Fluorescein 548;3,6-DICHLOROFLUORAN;2,7-Dichorofluorescin;DICHLOROFLUORESCEIN TS;FLUORESCEIN DICHLORIDE;dcorange25(fluorescein;DICHLORO (R) FLUORESCEIN
CBNumber:
CB0690319
???:
C20H10Cl2O5
??? ??:
401.2
MOL ??:
76-54-0.mol
MSDS ??:
SDS

2'',7''-??????????? ??

???
280 °C (dec.)(lit.)
?? ?
514.77°C (rough estimate)
??
0.79 g/mL at 20 °C
???
1.5300 (estimate)
???
12°C (53.6°F)
?? ??
room temp
???
???? ??
?? ?? (pKa)
4.46(at 25℃)
??? ??
?? ??
??
???~???, ??
pH ??
4.0 (colorless) - 6.6 (green)
???
???? ???? ?????. ??? ????????. ?? ???.
?? ??(λmax)
509nm
BRN
58009
???
????. ?? ???? ???? ????.
?? ??
Electroluminescent devices, sol-gel silica films, integrated circuits, photoresists, lithographic printing plates, recording material, LED devices, photoconductors, Solid-state dye laser, lithographic printing plates, inks, Fluorescent pH sensors, emissive probes, chemotherapeutic treatment of disease, phototherapeutic treatment of disease, photodynamic treatment of disease
InChIKey
NZVMJBGGAQSNLI-UHFFFAOYSA-N
CAS ??????
76-54-0(CAS DataBase Reference)
EPA
??
  • ?? ? ?? ??
  • ?? ? ???? ?? (GHS)
??? ?? Xi,F
?? ???? ?? 36/37/38-36-67-11
????? 26-36-16
????(UN No.) UN 1219 3/PG 2
WGK ?? 3
RTECS ?? NT8050000
TSCA Yes
HS ?? 3204 19 00
???? ?? KE-10142
????(GHS): GHS hazard pictograms
?? ?: Warning
??·?? ??:
?? ??·?? ?? ?? ?? ?? ?? ? ?? ?? P- ??
H315 ??? ??? ??? ????? ?? ????? ?? 2 ?? GHS hazard pictograms P264, P280, P302+P352, P321,P332+P313, P362
H319 ?? ?? ??? ??? ?? ? ?? ?? ??? ?? ?? 2A ?? GHS hazard pictograms P264, P280, P305+P351+P338,P337+P313P
??????:
P264 ?? ??? ?? ??? ????.
P264 ?? ??? ?? ??? ????.
P280 ????/???/???/?????? ?????.
P305+P351+P338 ?? ??? ? ?? ?? ???? ????. ???? ?????? ?????. ?? ????.
P337+P313 ?? ?? ??? ???? ???? ??· ??? ????.
NFPA 704
1
2 0

2'',7''-??????????? C??? ??, ??, ??

??? ??

red to yellow solid

??

2',7'-Dichlorofluorescein is used as an indicator and as a cellular antioxidant activity. It is also used for the determination of carbohydrates and hydrogen peroxide by fluorescence densitometry and chemiluminescence respectively. It is used as an indicator for argentometry by Fajans method. It is also involved in the cellular antioxidant activity assay. It is utilized as an additive in fluorescence and laser studies.

??

2',7'-dichlorofluorescein is a member of 2-benzofurans. It has a role as a fluorochrome. It derives from a fluorescin.

?? ??

2',7'-dichlorofluorescein has been used as an oxidation-sensitive fluorescent probe to measure ROS (reactive oxygen species) formation in cells.

???? ??

2',7'-dichlorofluorescein is an oxidation-sensitive fluorescent probe. 2′7′-dichlorofluorescein diacetate is oxidized to 2′7′-dichlorofluorescein in the presence of ROS (reactive oxygen species).

?? ??

[1] Chander Hans. “2’,7’-Dichlorofluorescein (DCF) or 2’,7’-dichlorodihydrofluorescein diacetate (DCFH2-DA) to measure reactive oxygen species in erythrocytes.” Biomedicine & Pharmacotherapy (2021): 111512.

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2'',7''-??????????? ?? ??:

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