IDH1 R132H mutant (human recombinant) Chemische Eigenschaften,Einsatz,Produktion Methoden
Beschreibung
Isocitrate dehydrogenases (IDHs) are NAD
+ and NADP
+-
dependent enzymes that catalyze the third step of the tricarboxylic acid cycle. IDHs catalyze oxidative decarboxylation of isocitrate producing α-
ketoglutarate (α-
KG) and carbon dioxide. IDH1 (cytosolic) and IDH2 (mitochondrial) are NADP
+-
dependent enzymes that catalyze reversible reactions. The IDH3 isoform, a NAD
+-
dependent multisubunit enzyme, is irreversible and allosterically regulated by a variety of positive (calcium, ADP, and citrate) and negative (ATP, NADH, and NADPH) effectors. IDH1 and IDH2 are mutated in >70% of lower grade gliomas. The most common IDH mutation, Arg132His, imparts new gain of function catalytic activity leading to the NADPH-
dependent conversion of α-
KG to 2-
hydroxyglutarate. Astrocytes expressing IDH1 R132H mutant have been shown to produce markedly increased levels of the R-
2-
hydroxyglutarate enantiomer, leading to transformation of cells through the HIF prolyl 4-
hydroxylase, EGLN.
IDH1 R132H mutant (human recombinant) Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
