376382-11-5
376382-11-5 結構式
基本信息
化合物 T14779
BRD 7389 (BRD7389)
BRD7389 >=98% (HPLC)
Insulin Expression Inducer, BRD7389
1-Phenethylamino-3H-naphtho[1,2,3-de]quinoline-2,7-dione
1-[(2-Phenylethyl)amino]-3H-naphtho[1,2,3-de]quinoline-2,7-dione
1-[(2-Phenylethyl)amino]-naphtho-3H-[1,2,3-de]quinoline-2,7-dione
Insulin Expression Inducer, BRD7389 - CAS 376382-11-5 - Calbiochem
3H-Naphtho[1,2,3-de]quinoline-2,7-dione, 1-[(2-phenylethyl)aMino]-
物理化學性質
常見問題列表
|
RSK1 1.5 μM (IC 50 ) |
RSK2 2.4 μM (IC 50 ) |
RSK3 1.2 μM (IC 50 ) |
CDK5/p35 6.5 μM (IC 50 ) |
DRAK1 2.8 μM (IC 50 ) |
FLT3 3.5 μM (IC 50 ) |
PIM1 3.7 μM (IC 50 ) |
PKG1α 6.5 μM (IC 50 ) |
SGK 13.8 μM (IC 50 ) |
BRD7389 (0.425-6.8 μM) induces insulin expression in mouse α-cells after 3 days treatment. BRD7389 induces a dose-dependent up-regulation of insulin (Ins2) mRNA, peaking at 0.85 μM; 5 days treatment with BRD7389 results in greater induction of insulin gene expression, about 50-fold at 0.85 μM.
BRD7389 (0.85-6.8μM) significantly up-regulates Pdx1 mRNA expression in mouse α-cell line.
BRD7389 also increases β-cell-specific gene expression in primary human islet cells.
BRD7389 (1 μM; added 30 min prior to Carbachol treatment 48 h) fully abolishes carbachols timulated cell proliferation, but has little effect on the basal level of proliferation.
RT-PCR
| Cell Line: | Mouse α-cell line |
| Concentration: | 0.425, 0.85, 1.7, 3.4, 6.8 μM |
| Incubation Time: | 3 days and 5 days |
| Result: | Up-regulated expression of Pdx1. |
Cell Proliferation Assay
| Cell Line: | SNU-407 colon cancer cell |
| Concentration: | 1 μM |
| Incubation Time: | Added 30 min prior to Carbachol treatment (48 h) |
| Result: | Almost completely blocked Carbachol (1 mM)-stimulated cell proliferation. |