3148-09-2
3148-09-2 結(jié)構(gòu)式
基本信息
中文別名
疣孢菌素疣孢菌素A
英文別名
veraVerrucarin
NSC 126728
VERRUCARIN A
MUCONOMYCIN A
Muconomycin A, NSC 126728
16a,21-trimethyl--stereoisomer
verrucarin A from myrothecium sp
n-17(18h),2’-oxirane)-3,9,14-trione,4,5,6,7,16,16a,19a,22-octahydro-4-hydroxy
spiro(16,18-methano-1h,3h,23h-(1,6,12)trioxacyclooctadecino(3,4-d)(1)benzopyra
物理化學(xué)性質(zhì)
熔點(diǎn)>360° (dec)
比旋光度D23 +206° (c = 1.012 in chloroform)
沸點(diǎn)512.66°C (rough estimate)
密度1.1922 (rough estimate)
折射率1.5500 (estimate)
儲存條件−20°C
溶解度Dichloromethane: soluble,DMSO: soluble,Ethanol: soluble,Methanol: soluble
酸度系數(shù)(pKa)12.76±0.70(Predicted)
形態(tài)固體
顏色White to light yellow
安全數(shù)據(jù)
警示詞危險(xiǎn)
危險(xiǎn)性描述H300+H310+H330
危險(xiǎn)品標(biāo)志T+
危險(xiǎn)類別碼26/27/28
安全說明22-36/37/39-45
危險(xiǎn)品運(yùn)輸編號UN 2811 6.1/PG 1
WGK Germany3
RTECS號WH1314900
危險(xiǎn)等級6.1(a)
包裝類別I
海關(guān)編碼2941900000
毒性LD50 in mice, rats, rabbits (mg/kg): 1.5, 0.87, 0.54 i.v. (Rüsch, Sthlin)
常見問題列表
生物活性
Verrucarin A (Muconomycin A) 是一種 D 型大環(huán)真菌毒素，來源于Myrothecium verrucaria，是一種蛋白質(zhì)合成 (protein synthesis) 的抑制劑。Verrucarin A 抑制白血病細(xì)胞系生長，激活巨噬細(xì)胞 caspases、凋亡和炎癥信號。Verrucarin A 能有效提高 p38 MAPK 的磷酸化，降低 ERK/Akt 的磷酸化。Verrucarin A 通過 p21 和 p53 的誘導(dǎo)引起細(xì)胞周期調(diào)控的解除。體外研究
Verrucarin A (0-0.6 μM/ml; 24-48 hours)-induces time- and dose-dependent growth inhibition in MCF-7 cells.
Verrucarin A increases the levels of reactive oxygen species (ROS), and subsequently induces mitochondrial membrane potential (Δψm) loss, leading to the increase of Bax/Bcl-2 ratio, cytochrome c release, caspase activation, PARP degradation, and apoptosis.
Cell Viability Assay
| Cell Line: | MCF-7 cells |
| Concentration: | 0-0.6 μM/ml |
| Incubation Time: | 24 and 48 hours |
| Result: | Growth of MCF-7 cells is significantly inhibited in a dose- and time-dependent manner, with the IC 50 s of 0.41 and 0.29 μM/ml for 24- and 48-h treatment periods, respectively. |