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25535-16-4

中文名稱 碘化丙啶
英文名稱 Propidium iodide
CAS 25535-16-4
EINECS 編號 247-081-0
分子式 C27H34I2N4
MDL 編號 MFCD00011921
分子量 668.39
MOL 文件 25535-16-4.mol
更新日期 2024/12/03 16:47:19
25535-16-4 結構式 25535-16-4 結構式

基本信息

中文別名
碘化丙啶
碘化酶
英文別名
3,8-DIAMINO-5-(3-DIETHYLAMINOPROPYL)-6-PHENYLPHENANTHRIDINIUM IODIDE METHIODIDE
3,8-DIAMINO-5-(3-DIETHYL-AMINOPROPYL)-6-PHENYLPHENANTHRIDIUM IODIDE METHIODIDE
3,8-DIAMINO-5-(3-[DIETHYLMETHYLAMMONIO])-6-PHENYLPHENAN-THRIDINIUM DIIODIDE
3,8-DIAMINO-5-[3-(DIETHYLMETHYLAMMONIO)PROPYL]-6-PHENYLPHENANTHRIDINIUM
3,8-DIAMINO-5-[3-(DIETHYLMETHYLAMMONIO)PROPYL]-6-PHENYLPHENANTHRIDINIUM DIIODIDE
PHENANTHRIDINIUM, 3,8-DIAMINO-5-[3-(DIETHYLMETHYLAMMONIO)PROPYL]-6-PHENYL-, DIIODIDE
PI
PROPIDIUM IODIDE
3,8-diamino-5-(3-(diethylmethylammonio)propyl)-6-phenyl-phenanthridiniudii
3,8-diamino-5-(3-(diethylmethylammonio)propyl)-6-phenylphenanthridiniumdiiod
3,8-diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridiniumiodidemethiodi
Propidium iodide solution
Propidium diiodide
PROPIDIUM IODIDE 95-98%
PROPIDIUM IODIDE, FOR FLUORESCENCE
PROPIDIUM IODIDE 95%
-Cellstain-PiSolution
-Cellstain-Pi
PROPIDIUM IODIDE RED POWDER
3,8-Diamino-5-[3-(diethylmethylamino)propyl]-6-phenylphenanthridium,diiodide
所屬類別
生物化工:其他生化試劑

物理化學性質

熔點220-225 °C (dec.) (lit.)
熔點220-225 °C (dec.)(lit.)
密度1.5072 (estimate)
儲存條件2-8°C
儲存條件2-8°C
溶解度難溶于水
形態(tài)片狀粉末
顏色深紅
水溶解性Soluble in water at 1mg/ml
敏感性Light Sensitive & Hygroscopic
最大波長(λmax)493 nm, 535 nm
BRN3843838
生物領域應用Detecting nucleic acids,cancer cells,spores,activity of hydrolase,microorganisms; apoptosis assay; measuring beta-amyloid in Alzheimer’s disease (AD); treating diseases associated with androgens,cancer
主要應用Electroluminescent displays; photoresists

安全數據

危險性符號(GHS)GHS hazard pictograms
GHS08
警示詞警告
危險性描述H341
危險品標志Xi,Xn
危險品標志Xi
危險類別碼R36/37/38
安全說明26-36-22-36/37
安全說明S26
WGK Germany3
WGK Germany3
RTECS號SF7949600
海關編碼29339900

應用領域

用途一
碘化丙啶(PI)是一種溴化乙啶的類似物,它在嵌入雙鏈DNA后釋放紅色熒光。盡管PI不能通過活細胞膜,但卻能穿過破損的細胞膜而對核染色。PI經常被用 來與Calcein-AM或者FDA等熒光化合物一起使用,能同時對活細胞和死細胞染色。

常見問題列表

簡介

碘化丙啶是一種可對DNA染色的細胞核染色試劑,常用于細胞凋亡檢測,英文全稱是Propidium Iodide。它是一種溴化乙啶的類似物,在嵌入雙鏈DNA后釋放紅色熒光。

生物活性
Propidium Iodide 是可用于細胞染色的紅色熒光染料。
體外研究

Propidium Iodide is a cell-membrane impermeable dye with characteristic excitation maximum at 535 nm and emission maximum at 617 nm which intercalates with nucleic acids with a stoichiometry of one dye per 4-5 base pairs with little sequence preference. Propidium Iodide has evidenced of having no toxic effects on neurons, being today’s most common marker for membrane integrity and cell viability when applied prior to fixation (pre-fixation Propidium Iodide staining method). The pre-fixation staining has been widely used for quantitative assessments of neuronal cell decline in models of acute neurodegeneration, visualized as intensely labeled PI + -pycnotic nuclei of degenerating neurons . Propidium Iodide cannot cross the membrane of live cells, making it useful to measure the percentage of apoptotic cells by flow-cytometric analysis. The flow cytometric data shows an excellent correlation with the results obtained with both electrophoretic and colorimetric methods. This new rapid, simple and reproducible method proves useful for assessing apoptosis of specific cell populations in heterogeneous tissues such as bone marrow, thymus and lymph nodes.

知名試劑公司產品信息

碘化丙啶價格(試劑級)
報價日期產品編號產品名稱CAS號包裝價格
2024/11/11XW2553516403碘化丙錠25535-16-45MG127元
2024/11/11XW2553516402碘化丙錠25535-16-425MG329元
2024/11/11XW2553516401碘化丙錠25535-16-4100MG906元
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