| Identification | More | [Name]
HEPES | [CAS]
7365-45-9 | [Synonyms]
2-[4-(2-HYDROXYETHYL)-1-PIPERAZINE]ETHANESULFONIC ACID
2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID
4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID
4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID
GOOD'S BUFFER
HEPES
HEPES-ACID
HEPES BUFFER
HEPES BUFFERED SALINE
HEPES BUFFER PH 7.5
HEPES BUFFER PH 8.0
LABOTEST-BB LT00138109
LABOTEST-BB LT00233156
N-(2-HYDROXYETHYL)PIPERAZINE N-2-ETHANESULFONIC ACID
N-2-HYDROXYETHYLPIPERAZINE-N'-2-ETHANESULFONIC ACID
N-2-HYDROXYETHYLPIPERAZINE-N'-2-ETHANE SULPHONIC ACID
4-(2-hydroxyethyl)-1-piperazineethanesulfonicaci
hepesfreeacid
N-(2-Hydroxyethyl)piper
4-(2-Hydroxyethyl)-1-piperazineethanesulphonic acid | [EINECS(EC#)]
230-907-9 | [Molecular Formula]
C8H18N2O4S | [MDL Number]
MFCD00006158 | [Molecular Weight]
238.3 | [MOL File]
7365-45-9.mol |
| Chemical Properties | Back Directory | [Appearance]
White crystalline powder | [Melting point ]
234-238 °C
| [Boiling point ]
408℃[at 101 325 Pa] | [bulk density]
560kg/m3 | [density ]
1.07 g/mL at 20 °C
| [vapor pressure ]
0Pa at 25℃ | [refractive index ]
n20/D 1.339
| [storage temp. ]
2-8°C
| [solubility ]
H2O: 1 M at 20 °C, clear, colorless
| [form ]
powder
| [color ]
White | [PH]
5.0-6.5 (1Maqueous solution) | [PH Range]
7.0 - 7.6 | [pka]
7.5(at 25℃) | [Stability:]
Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture. | [biological source]
synthetic | [Water Solubility ]
Soluble | [λmax]
λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.04 | [Detection Methods]
T,NMR | [Merck ]
14,4654 | [BRN ]
883043 | [InChIKey]
JKMHFZQWWAIEOD-UHFFFAOYSA-N | [LogP]
-3.85 at 20℃ | [CAS DataBase Reference]
7365-45-9(CAS DataBase Reference) | [EPA Substance Registry System]
7365-45-9(EPA Substance) | [Absorption]
≤0.05 at 290 in H2O at 33% |
| Safety Data | Back Directory | [Hazard Codes ]
Xi | [Risk Statements ]
R36/37/38:Irritating to eyes, respiratory system and skin . | [Safety Statements ]
S24/25:Avoid contact with skin and eyes .
S22:Do not breathe dust .
S36:Wear suitable protective clothing .
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice . | [WGK Germany ]
1
| [RTECS ]
TL6809000
| [TSCA ]
Yes | [HS Code ]
29335995 |
| Hazard Information | Back Directory | [Description]
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. | [Chemical Properties]
White crystalline powder | [Uses]
Biological buffer. | [Uses]
Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability. | [Definition]
ChEBI: A HEPES that is ethanesulfonate substituted by a 4-(2-hydroxyethyl)piperazin-4-ium-1-yl group at position 2. | [General Description]
HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions. | [Biological Activity]
Multi purpose buffer used in biological research. | [storage]
Room temperature | [Purification Methods]
Crystallise the acid from hot EtOH and water. It is a useful buffer. [Beilstein 23 V 376.] |
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