| Identification | More | [Name]
1-Methoxy-5-methylphenazinium methyl sulfate | [CAS]
65162-13-2 | [Synonyms]
1-METHOXY-5-METHYLPHENAZINE METHOSULFATE
1-METHOXY-5-METHYLPHENAZINIUM METHYL SULFATE
1-METHOXYPHENAZINE METHOSULFATE
1-METHOXY PMS
N-METHYL METHOXYPHENAZINE-METHOSULFATE
1-methoxy-5-methyl-phenaziniumethylsulfate
1-Methoxy PMS N-Methyl methoxyphenazinemethosulfate
1-methoxy-5-methylphenazinium methyl sulphate
MOPMS
Methoxyphenazine-methosulfate
1-METHOXY-5-METHYLPHENAZINIUM METHYLSULFATE (1-METHOXY PMS)
1-Methoxy-5-methyl-5-phenazinium·methylsulfate | [EINECS(EC#)]
265-579-6 | [Molecular Formula]
C15H16N2O5S | [MDL Number]
MFCD00040648 | [Molecular Weight]
336.36 | [MOL File]
65162-13-2.mol |
| Chemical Properties | Back Directory | [Melting point ]
172 °C | [storage temp. ]
Inert atmosphere,Room Temperature | [solubility ]
Water: 1 mg/ml | [form ]
powder to crystal | [color ]
Orange to Brown to Dark purple | [InChI]
InChI=1S/C14H13N2O.CH4O4S/c1-16-11-7-4-3-6-10(11)15-14-12(16)8-5-9-13(14)17-2;1-5-6(2,3)4/h3-9H,1-2H3;1H3,(H,2,3,4)/q+1;/p-1 | [InChIKey]
MASUWVVNWALEEM-UHFFFAOYSA-M | [SMILES]
S([O-])(=O)(=O)OC.O(C1=CC=CC2=[N+](C3=CC=CC=C3N=C12)C)C | [CAS DataBase Reference]
65162-13-2(CAS DataBase Reference) |
| Safety Data | Back Directory | [Hazard Codes ]
Xn | [Risk Statements ]
R22:Harmful if swallowed.
R36/38:Irritating to eyes and skin .
R40:Limited evidence of a carcinogenic effect. | [Safety Statements ]
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice . | [WGK Germany ]
3 | [HS Code ]
2933.99.8290 |
| Hazard Information | Back Directory | [Uses]
1-Methoxy-5-methylphenazinium methyl sulfate (PMS) has a redox potential of +63 mV. PMS is often used as an electron carrier for NADH tetrazolium and is a useful reagent for NAD(P)H-tetrazolium-based analytical systems. It is mainly used in the cytotoxic proliferation reagent CCK-8 experiment.
| [Mechanism of action]
1-Methoxy-5-methylphenazinium methyl sulfate contains WST-8 [chemical name: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfonic acid benzene)-2H-tetrazolyl monosodium salt], which is reduced to a highly water-soluble yellow formazan dye by dehydrogenase in cells under the action of the electron carrier 1-methoxy-5-methylphenazinium dimethyl sulfate. The amount of formazan generated is proportional to the number of living cells. Therefore, this property can directly analyze cell proliferation and toxicity. The main uses of this reagent are drug screening, cell proliferation assay, cytotoxicity assay, and tumour drug sensitivity test. | [storage]
Store at -20°C, protect from light |
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