ChemicalBook CAS DataBase List (S)-1-Benzyl-3-pyrrolidinol

(S)-1-Benzyl-3-pyrrolidinol synthesis

6synthesis methods

Product Name:(S)-1-Benzyl-3-pyrrolidinol
CAS Number:101385-90-4
Molecular formula:C11H15NO
Molecular Weight:177.24

775-16-6 Synthesis

775-16-6
321 suppliers
$31.80/1gm:

101385-90-4
302 suppliers
$12.00/1g

Yield:101385-90-4 92%

Reaction Conditions:

with recombinant Rhodococcus erythropolis DSM 43297 ketoredutase;nicotinamide adenine dinucleotide in aq. phosphate buffer;isopropyl alcohol; pH=7.0 at 50; for 21 h;Enzymatic reaction;stereoselective reaction;

Steps:

2.4. General procedure for biotransformation
General procedure: The reaction mixture containing 200 mM substrate, 1mM NAD⁺, 5% (v/v) 2-propanol and 10mg crude enzyme READH in 1mL potassium phosphate buffer (100mM, pH 7.0) was incubated at 50 °C. For ChKRED20, 40% (v/v) 2-propanol and a reaction temperature of 40 °C were applied instead. The reaction was monitored by TLC, and terminated by extracting with methyl tert-butyl ether (1 mL). The organic extract was dried over anhydrous sodium sulfate and concentrated. The samples were subjected to chiral HPLC to determine the conversion and enantiomeric excess. The products were purified by silica gel column chromatography, and identified by NMR analysis, optical rotation measurements and mass spectrometry.

References:

Li, Chao;Liu, Yan;Pei, Xiao-Qiong;Wu, Zhong-Liu [Process Biochemistry,2017,vol. 56,p. 90 - 97]

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