ChemicalBook CAS DataBase List Lactulose

Lactulose synthesis

3synthesis methods

Product Name:Lactulose
CAS Number:4618-18-2
Molecular formula:C12H22O11
Molecular Weight:342.3

63-42-3 Synthesis

63-42-3
538 suppliers
$36.00/100g

4618-18-2
402 suppliers
$28.00/1g

Yield:-

Reaction Conditions:

with β-galactosidase from kluyveromyces lactis;glucose isomerase from streptomyces rubiginosus in aq. phosphate buffer; pH=7.5 at 53.5; for 3 h;Catalytic behavior;Enzymatic reaction;Temperature;Concentration;

Steps:

Lactulose synthesis
The following reaction conditions were used for lactulose synthesis with the immobilized enzymes: lactose (14%, 16%, 18%, and 20%, w/v), temperature (47, 50, 53.5, 57, and 60 °C), ionic strength (50, 100, 150, 200, 250, and 300 mM) of sodium phosphate buffer at pH 7.5, ratio (1:1, 1:2, 1:3, 1:4, 1:5, and 1:6) of immobilized enzymes, agitation speed of 150 rpm, and a reaction time of 3 h. The lactose conversion rate (defined as ([S₀]-[S_s])/[S₀] × 100 %) and specific productivity (defined as [P]/([E] × t), mg/U h) were calculated. To test the reusability of the immobilized enzymes, they were filtered and washed with 100 mM sodium phosphate buffer (pH 7.5) between batch reactions. The relative catalytic activity of the immobilized enzymes was calculated as the lactulose ratio of the reused batch to the first batch, and was expressed as a percentage. All experiments were conducted in duplicate, and data were represented as an error bar.

References:

Song, Yoon-Seok;Lee, Hee-Uk;Park, Chulhwan;Kim, Seung-Wook [Carbohydrate Research,2013,vol. 369,p. 1 - 5]

FullText

57-48-7 Synthesis