Cytidine synthesis
- Product Name:Cytidine
- CAS Number:65-46-3
- Molecular formula:C9H13N3O5
- Molecular Weight:243.22
Yield:65-46-3 0.7%
Reaction Conditions:
with phosphoric acid;magnesium sulfate in water at 20; under 760.051 Torr;
Steps:
General procedure: The aqueous solution of 15 mM D-ribose, 15 mM phosphoric acid, and 5 mM nucleobases was injected with 3 mM magnesium sulfate by a mechanical syringe pump through a hypodermic needle to the fused silica capillary directing toward a mass spectrometer inlet. A coaxial sheath gas (dry N2 at 120 psi) flow around the capillary results in nebulization and also helps to direct the spray emerging from the capillary tip toward the mass spectrometer inlet (the flow rate of 5 μL/min through silica tubing). A positive voltage, +5 kV, was applied to the hypodermic needle. At a mass spectrometer inlet, the reactants,intermediates, and products are released from droplets by Coulomb fission and enter into the mass spectrometer through a heated capillary. The capillary temperature was maintained at 275 °C and the capillary voltage at44 V. To confirm the identities of synthesized ribonucleosides, tandem MSwas conducted by CID. For all mass spectrometric analyses, the spray distance(the distance from spray tip to the entrance of the heated capillary) was kept at 5 mm. Mass spectra were detected by high-resolution MS (Thermo ScientificLTQ Orbitrap XL Hybrid Ion Trap-Orbitrap). All of the necessary chemicals were purchased from Sigma-Aldrich. HPLC-grade solvents were purchased from Fisher Scientific.
References:
Nam, Inho;Nam, Hong Gil;Zare, Richard N. [Proceedings of the National Academy of Sciences of the United States of America,2018,vol. 115,# 1,p. 36 - 40]
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