For the blood anticoagulants between EDTA K2 and K3, if not very well understand their people are generally unable to distinguish between them what difference. As a manufacturer with more than ten years of experience in R & D and production of anticoagulants, Desheng is obliged to show you the knowledge you may not know.

Appearance:

EDTA K3 and EDTA K2 are pure white powder, but EDTA K3 powder will be more delicate and the water soluble effect will be better than EDTA K2. Of course, this kind of naked eye can not be seen, but the comparison in a large number of experiments is still more significant.

Use:

In fact, EDTA K3 and EDTA K2 are not very different, both of which are salt of EDTA. However, the ions are different. One is with two potassium ions, one with three potassium ions, and the other is EDTA. The specific difference is as follows:

● EDTA K3 pH value is 6.8-7.0 neutral and does not affect the pH value of blood. At the same time, the ability of complexation calcium ion is stronger than EDTA K2, and the anticoagulation effect is stronger than EDTA K2;

● if EDTA concentration increases, EDTA K3 is more likely to cause the shrinkage of red blood cells compared with EDTA K2（ When EDTA K3 concentration reaches 7.5mg/ml, 11% of the red blood cells will shrink;

● the solubility of EDTA K2 is lower than K3, sometimes it will affect the anticoagulation effect.

● if the specimen is placed for a period of time, EDTA K3 is more likely to cause the cell volume increase compared with EDTA K2 (4 hours, volume increase 1.6%)

● EDTA K3 is more likely to reduce the mean red cell volume (MCV) compared with EDTA K2 (generally, there is a negative deviation of -0.1 ~ -1.3% compared with EDTA K2)

● if EDTA K3 is used as a liquid additive, it will cause dilution of the sample. All direct test items, such as hemoglobin, red blood cells, white blood cells and platelet count, should be 1-2% lower than EDTA K2 as anticoagulant.

● with some analytical instruments, EDTA K3 will decrease the leukocyte count at higher concentrations.