Hepes Chinese name: 4-(2-hydroxyethyl)-1-piperazine ethane sulfonic acid, which belongs to a kind of two-ion sulfonic acid buffer and one of Good buffers. Hepes is widely used in cell culture. Due to the growth and metabolism of cells in culture, the environmental pH value of the culture medium will change drastically. Therefore, in order to maintain an environment where cells can be cultured stably, it is necessary to add an appropriate amount of certain biological buffers. However, there are so many biological buffers and alternatives on the market that can be used for cell culture, why use Hepes?

Generally, the optimal culture environment for most cells is pH 7.2-pH 7.4, and the buffer range of Hepes is just within pH 6.8-pH 8.2. This is beneficial for cell culture. Compared with other buffers, Hepes has higher stability in maintaining the pH value of cell culture medium. This is also Hepes is widely used in cell culture, tissue culture, protein purification and extraction, and immunity. Reasons for precipitation, cell lysis, live cell imaging and other biological/biochemical research.

Hepes produces hydrogen peroxide when exposed to ambient light. It is not yet certain whether it is a problem in the bicarbonate cell culture buffer. Therefore, it is recommended to put the HEPES-containing solution in the dark as much as possible to prevent oxidation.

Nowadays, biological buffers are often used in biochemical experiments, which has led to a very large market demand for biological buffers. Therefore, buffer merchants on the market are mixed, making procurement very difficult. Recommend a manufacturer here: Hubei Xindesheng Material Co., Ltd.

As a professional manufacturer of biological buffers, Desheng provides high-quality Hepes, and also produces a series of biological buffers such as Tris, Mops, and Taps. In addition, Desheng company can also provide special testing services and after-sales service according to customers' applications. In any case, Desheng is the best choice to buy Hepes. If you are interested in the company's products, you can enter the company's official website to contact customer service for more information.