Tris HCl is also called Tris hydrochloride. Tris is widely used. Tris buffer is not only used as a solvent for nucleic acids and proteins, but also has many important uses.

 

Tris was used for protein crystal growth under different pH conditions. The low ionic strength of Tris buffer can be used for the formation of intermediate fibers of lamin. Tris is also one of the main components of protein electrophoretic buffer, which forms a buffer system with glycine in the electrophoretic buffer to stabilize the pH in the process of electrophoresis. In addition, Tris is an intermediate for the preparation of surfactants, vulcanization accelerators and some drugs. Tris is also used as a titration standard. As a protein buffer, Tris is not suitable to be used if mass spectrometry is needed in the follow-up work. Try to replace it with a buffer that can be tolerated by other mass spectrometers.

 

Tris HCl is obtained from Tris as the main raw material, and its solution is acidic. It is mainly used as biological buffer to adjust the pH value of virus preservation solution. If you need to adjust to neutral or alkaline, you can add Tris or NaOH to achieve. Apart from this information, what are the configuration methods of Tris HCl? How much do you know? The configuration of Tris HCl is described in the following.

 

The preparation method of Tris HCl buffer was as follows

 

1、Tris-Hcl（0.05mol/L，25℃）

 

Mix 50 ml of 0.1 mol / L Tris solution with X ml of 0.1 mol / L hydrochloric acid, and then dilute with water to 100 ml

 

PH x/ml PH x/ml

 

7.1 45.7 8.1 26.2

 

7.2 44.7 8.2 22.9

 

7.3 43.4 8.3 19.9

 

7.4 42.0 8.4 17.2

 

7.5 40.3 8.5 14.7

 

7.6 38.5 8.6 12.4

 

7.7 36.6 8.7 10.3

 

7.8 34.5 8.8 8.5

 

7.9 32.0 8.9 7.0

 

The molecular weight of Tris was 121.14:0.1mol/l, and the solution was 12.114g/l. Tris solution can absorb carbon dioxide from the air, so the bottle should be tightly closed. When Tris HCl solutions with different concentrations and pH values are prepared, a certain pH value of 0.05mol/l solution can be prepared first, and then diluted to the required concentration.

 

2、1M Tris-Hcl（PH7.4、PH7.6、PH8.0）

 

Component concentration 1m Tris HCl

 

Configuration quantity 1L

 

Configuration method:

 

1、 Weigh 121.1g Tris and put it in a 1 ﹣ l beaker;

 

2、 Add about 800 ml of deionized water and fully stir to dissolve;

 

3、 Add concentrated HCl according to the following value to adjust the required pH value;

 

PH value concentrated HCl

 

7.4 about 70 ml

 

6, about 60ml

 

0, about 42ml

 

4、 Volume the solution to 1 L;

 

5、 After high temperature and high pressure sterilization, store at room temperature.

 

3、1.5M Tris-Hcl（PH8.8）

 

Component concentration 1.5m Tris HCl

 

Configuration quantity 1L

 

Configuration method:

 

Weigh 181.7g Tris and put it in 1L beaker;

 

Add about 800 ml of deionized water and fully stir to dissolve;

 

Adjust pH value to 8.8 with concentrated HCl;

 

Volume the solution to 1 L;

 

After high temperature and high pressure sterilization, store at room temperature.

 

It should be noted that the pH value of Tris solution should be adjusted after the solution is cooled to room temperature, because the pH value of Tris solution varies greatly with the temperature. The pH value of Tris solution decreases by about 0.03 unit when the temperature increases by 1 ℃.

 

The above is the configuration method of Tris HCl provided by Hubei xindesheng Material Co., Ltd. In addition to Tris HCl, Desheng also produces other chemicals, such as blood vessel additives, enzyme preparations, chemiluminescence reagents, biological buffers, chromogen substrates and carbomer. At present, Desheng company has been established for decades, has its own R & D team, and has invested a lot of energy in biological buffers. Reliable product quality, price concessions can be discussed, interested in understanding, you can call consultation, Desheng welcome your call.