Chemiluminescence is the light radiation produced by the absorption of chemical energy by a certain substance molecule. For a chemical reaction to become a luminescent reaction, two conditions must be met: first: the reaction must provide sufficient energy; second, the chemical energy must be able to be This kind of substance molecules absorb and generate electronic excited states, and have sufficient fluorescence quantum yield. So far, most of the chemiluminescence reactions studied are redox reactions, and most of them are liquid-phase chemiluminescence reactions. Luminol is one of the chemiluminescence reagents, and its luminous efficiency can reach 0.01. The luminescence mechanism of luminol and its derivatives are summarized as follows.

What are the derivatives of luminol

Luminol's derivatives mainly include isoluminol, 4-aminohexyl-N-ethyl isolunol, AHEI and ABEI and so on. Luminol can be oxidized by some oxidants under alkaline conditions and undergo a chemiluminescence reaction, radiating chemiluminescence with a maximum emission wavelength of 425nm. Using the inhibitory effect of organic compounds on the chemiluminescence reaction of luminol, it is of great significance to determine the organic compounds that have a quenching effect on the chemiluminescence reaction.

Luminol can detect and analyze the concentration of metal ions

Under normal circumstances, the chemiluminescence reaction of luminol with hydrogen peroxide is quite slow, but the reaction is very rapid when certain catalysts are present. The most commonly used catalyst is metal ions. In a large concentration range, the concentration of metal ions is proportional to the luminescence intensity, so that chemiluminescence analysis of certain metal ions can be performed. This reaction can be used to analyze organic compounds containing metal ions to achieve Very high sensitivity.

Luminol can be labeled with carboxylic acid and ammonia compounds for detection

It is to label the derivatives of luminol such as isoluminol (ABEI) to carboxylic acid and ammonia compounds, and then after separation by high performance liquid chromatography (HPLC) or liquid chromatography (LC), and then under alkaline conditions It reacts with hydrogen peroxide-potassium ferricyanide for chemiluminescence detection. Other separation methods can also be used, such as labeling the newly synthesized chemiluminescence reagent isoluminol isothiocyanate to yeast RNA, separating it by centrifugation and dialysis, and then performing chemiluminescence detection. In addition, N 2 (B2 carboxyl propionyl) isoluminol was used, and its performance was studied.

Luminol reagent is not only used for biochemical detection, carboxylic acid and ammonia compound labeling, metal ion detection, but also for criminal investigation blood stain detection, with very high sensitivity. Luminol produced by Desheng is light yellow powder, which needs to be dissolved in lye when used.With cash, store away from light.