The main component of hepes buffer is hydroxyethylpiperazine ethanesulfonic acid. HEPES is a non-ionic amphoteric buffer, which has a good buffering capacity in the range of pH 7.2-7.4. It is widely used in cell culture media, protein, IVD kits and other fields. It adjusts the pH value of the solution in the range of 6.8 to 8.2, which is especially suitable for pH adjustment under physiological conditions. In addition to the above applications, it has also been used in cosmetic formulations. To
Most culture media contain phenol red as a pH indicator, acidic culture media is orange-yellow, alkaline culture media is dark red. HEPES is somewhat expensive and may be toxic to some cells at high concentrations. In principle, hepes buffer can be used Instead of bicarbonate, in order to remove the need for high-concentration CO2 culture environment restrictions. In practice, it is not so simple. Dissolved CO2 and bicarbonate are also important for good cell growth.
In cosmetic solutions, HEPES is used to balance the properties of its more acidic counterparts. Because HEPES can keep the pH constant during the chemical reaction, it is often paired with glycolic acid and other ingredients to help extend and maintain the quality of skin care products. Usually choose ingredients that provide exfoliation to improve the appearance of the skin.
Desheng Biochemical is a manufacturer specializing in the production of biological buffers, with a wide range of products. Since its establishment in 2005, it has more than ten years of R&D and production experience. The HEPES buffer produced by Desheng has high purity and wide application range. If necessary, please contact us!