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Solutions to the problems encountered in the use of blood collection tube heparin anticoagulant
Release time: 2021-10-18
Recently, with the approach of the Spring Festival, the prices of pigs, cattle and sheep have gradually increased. And our heparin products are mainly extracted from the small intestine and other tissues of animals such as pigs, cattle and sheep. The price of heparin itself is on the high side. When the prices of raw materials are rising, one can imagine the price of heparin. Therefore, when we use heparin, we must learn more about the impact on it in different environments, so that it is beneficial to us in terms of cost and time.
1. Can the heparin anticoagulation tube be placed directly after blood sampling?
Answer: No, in order to ensure sufficient blood anticoagulation, the heparin salt test tube must be inverted and mixed 5-8 times as soon as possible after the blood collection is completed. Especially when the blood collection environment temperature is higher than 25°C, the blood and heparin lithium must be thoroughly mixed in time, otherwise Easily lead to blood clotting or local clotting.
2. How long should it take to complete the test after blood collection for lithium heparin anticoagulation?
Answer: Heparin anticoagulation is not irreversible anticoagulation. Therefore, the test should be completed within 6 hours after blood collection in the lithium heparin anticoagulation test tube, otherwise it may cause errors in the test results.
3. What is the appropriate amount of heparin in the heparin tube?
Answer: Heparin may be involved in the metabolism of cell enzymes and ions, so the amount of heparin used may affect the test results. The added dosage of heparin should ensure the full and partial anticoagulation of the specimen, so as to ensure that most of the plasma indicators can be repeated within 6 hours, especially sensitive indicators such as AST, ALT, TBIL, DBIL, and GGT.
4. Can heparin be used with separating gel at the same time?
Answer: Lithium heparin can be used together with the separation gel. The anticoagulation effect, siliconization of the tube wall, centrifugal conditions, and separation gel quality will all affect the blood separation effect. It is recommended to use with the blood separation gel produced by our company to obtain high-quality plasma samples.
5. What factors will affect the titer of heparin?
Answer: The concentration of sodium chloride has an effect on the titer of heparin sodium; the amount of hydrogen peroxide has an effect on the titer of heparin sodium; and the oxidation time has an effect on the titer of heparin sodium.
6. Why do some lithium heparin tubes show tiny coagulation fragments after blood collection?
Answer: This is because the concentration of the local anticoagulant in the lithium heparin anticoagulant tube is too high, which causes part of the blood to enter the blood collection tube and cannot be fully mixed with the lithium heparin, which results in some tiny clots. This may be caused by the failure to invert and mix the test tube in time after blood sampling.
7. What are the storage conditions for lithium heparin?
Answer: This product can be sealed and stored in a cool and dry place, protected from light and moisture. The aqueous solution of this product can be sealed and stored at 0-4°C in a sterile state, and the maximum shelf life should not exceed 7 days. It is recommended that the aqueous solution of this product be used immediately after preparation, and do not store it in a solution state for a long time. It is forbidden to use heparin lithium when there are foreign matter, abnormal smell, color and effective shelf life.
8. Can the heparin solution be used when it is cloudy?
Answer: When the lithium heparin solution appears turbid, it may be that the lithium heparin solution has been infected or deteriorated. Please do not continue to use it.