3-Morpholine propane sulfonic acid, MOPS for short, is a zwitterionic buffer reagent commonly used in biochemistry and molecular biology. MOPS itself belongs to Good's buffer. It can not only be used as a buffer system for cell culture media, but also common The running buffer and protein purification chromatography buffer.
What kind of experiments can MOPS be used for?
1. As a running buffer in electrophoresis and protein purification in chromatography;
2. Formulated into a variety of agar media for the cultivation of bacteria, yeast and mammalian cells. However, the concentration higher than 20mM is not recommended for mammalian cells;
3. It can be used as a lysis buffer for E. coli cells;
4. As the eluent in gel filtration chromatography;
5. Used in Northern hybridization, as a buffer for RNA separation and membrane transfer;
6. Suitable for the determination of bicinchoninic acid (BCA);
7. Used in the study of electron transfer and phosphorylation of chloroplast thin layer preparation.
8. It can be used in biochemical diagnostic kits, such as DNA/RNA extraction kits, PCR kits, and creatinine determination kits.
MOPS transportation and storage
MOPS powder can be stored at room temperature for several years. However, once standing still, the bulk powder may gather together and form a hard mass, but its chemical properties will not be affected. The MOPS solution can be stored at 2-8°C for at least 6 months, filtered and sterilized with a 0.2 μm filter membrane, and autoclaving is not recommended. If you need to prepare a nuclease-free MOPS solution, you can autoclave the water first, and then add the powder to dissolve it
Features of MOPS buffer
(1) It has good solubility in aqueous solution, and the aqueous solution is colorless and transparent;
(2) It is chemically stable, non-toxic to organisms, and has no obvious penetrating effect on biological membranes;
(3) The acid-base dissociation constant (pKa value) is stable, generally 6-8, the ability to release protons is stable, and is less affected by ion concentration, solution composition and temperature;
(4) It is not a metabolite, activator or inhibitor of a biochemical reaction series, nor is it a substrate of an enzyme, that is, it does not react with a variety of enzymes and chemical reagents. When used in tissue culture, virus diagnosis and other tests, the cells show Higher vitality.
(5) There is actually no characteristic absorption in the ultraviolet wave, and only weak absorption when the wavelength is less than 230-240nm, so it does not interfere with the usual photometric measurement.
(6) No or weak complexing ability to cations;
Zwitterionic buffer has many advantages, is widely used, and has great market demand, and has been widely produced. Desheng Technology can provide a variety of high-quality zwitterionic buffers, including HEPES, MOPS, CAPS and other advantageous products. Among them, the quality of HEPES and MOPS products has been recognized and praised by customers in various industries.