HEPES (4-2-hydroxyethyl-1-piperazinyl-ethanesulfonic acid) is an organic substance used to prepare buffer solutions, widely used in cell culture to maintain a stable physiological pH value , Its pKa at 20°C is 7.55. HEPES is used to protect frozen enzyme solutions from freezing-induced pH changes. Taking into account the phototoxicity of HEPES (hydrogen peroxide will be generated if exposed to ambient light), this buffer should be stored in a darker environment after being configured as a solution to maintain its normal function during subsequent experiments.
HEPES is frequently used in cell culture because it has been proven to be a good maintainer of physiological pH. It has advantages over other buffers (such as carbonate buffers). In addition, it can also avoid carbon dioxide release caused by Caused by cell growth. The PH value of HEPES decreases with temperature, but the dissociation constant pK of phosphate and bicarbonate buffers hardly changes with temperature. HEPES behaves like water. When the temperature decreases, its dissociation constant will decrease. HEPES can be used to maintain the structure and function of the enzyme at low temperatures.
The HEPES produced by Desheng is white crystalline powder with good purity and high quality. The main content is ≥99.50%, iron ion ≤5ppm, sulfate ≤0.05%, and heavy metal residue (Pb) ≤10ppm. A small amount of trial sample powder can be provided, and it can be configured into a solution for testing.
As a manufacturer specializing in the production of biological buffers, it has 16 years of production research and development experience since its establishment in 2005. At present, the biological buffer products produced by Desheng include HEPES, MOPS, TRIS, TAPS, CAPS, BICINE, EPPS, PEP Wait for a series of biological buffers, please contact us for consultation if necessary!