Nowadays, the virus preservation solution used in the new coronavirus sampling tube on the market has different inactivation effects. A standard inactivated virus preservation solution is needed to ensure complete inactivation and stable nucleic acid, and effectively reduce false negatives.
At present, the form of prevention and control of the new coronavirus is still very severe. As the first step of nucleic acid detection, the importance of sample collection and preservation is undoubted. The inspection industry often says that "the garbage specimen yields garbage results". The most important influencing factor is the sample. collection. If there is a problem with the sample collection, how well the subsequent work is done, the final result is also invalid. At present, there are hundreds of virus collection tube manufacturers in China, many of which claim that "non-guanidine salt" and "inactivated" preservation solutions can really inactivate viruses? Can the viral RNA be truly preserved from degradation?
Now, a team has compared the effects of a preservation solution containing guanidine hydrochloride and guanidine isothiocyanate on the market with a preservation solution containing no guanidine salt in the preservation of poultry coronavirus at 37°C. It turns out that the main ingredient is the preservation solution of guanidine salt, and the preservation effect is very unsatisfactory!
Guanidine salt (guanidine isothiocyanate or guanidine hydrochloride, etc.), as a classic protein denaturant, is often used for cell lysis in nucleic acid extraction, and can also achieve the effect of inactivating viruses, but the guanidine salt component does not have the ability to perform under normal temperature conditions. The ability to preserve viral nucleic acid is very easy to cause sample degradation, so it is not suitable for the preservation of new coronavirus samples, so it is strongly recommended that you choose a virus preservation solution that does not contain guanidine salts.
The current nucleic acid detection is to detect the viral RNA in the sample, and the virus needs to be lysed. Therefore, as long as the integrity and stability of the viral nucleic acid can be ensured, there is no need to preserve the live virus. Therefore, in the current situation of fighting the new coronavirus epidemic, it is more ideal to inactivate the virus while collecting samples.
Since the outbreak of the epidemic, Hubei Xindesheng Materials Co., Ltd. has been devoted to the research and development of virus preservation solutions, striving to provide society with its own strength. After many experiments and tests, Desheng has been able to ensure that the product quality and experience are very up to standard. Among them, our company produces inactivated and non-inactivated virus preservation solutions, which can be adjusted and selected on demand. In addition, Desheng reminds our customers that when purchasing virus preservation solutions, you should not blindly pursue low prices. You can take samples and go back for testing and comparison. The suitable ones are good.