Biological buffer is a chemical substance that can stabilize the required range of pH. There are many types and different pH ranges. Therefore, it is widely used in various experiments. Adding buffers can change the pH in the experiment. Sex, which plays a vital role in the success of the experiment. In fact, biological buffers have not been widely used before 1996. Traditional buffers cannot effectively buffer pH values ??higher than pH 7.5, so they are limited to many applications.
In 1996, Dr. Norman Good developed zwitterionic buffers. These buffers can effectively solve some of the problems encountered in traditional buffers, because the series of biological buffers developed this time are relatively close to our physiological pH value, and It is non-toxic to cells and will not be absorbed through cell membranes. Among them, HEPES and PBS are two commonly used buffers.
Comparison of HEPES buffer and PBS buffer:
Effective PH value range
HEPES: 6.8-8.2 PBS: 5.8-8.0
Effect on cells
HEPES: It is non-toxic to cells. When the HEPES buffer system is used, the maximum cell density and viability can be seen.
PBS: Non-toxic to cells, cell culture samples diluted in PBS may inadvertently reduce cell viability.
Relationship with metal ions
HEPES: Does not produce complexes or precipitates with metal ions.
PBS: It will produce complexes or precipitates with certain metal ions.
Changes in freezing conditions
HEPES: The pH of the solution will not change under freezing conditions.
PBS: The pH of the solution will change significantly under freezing conditions.
Based on the above points, it is not difficult to see that HEPES buffer has certain advantages compared with PBS buffer. HEPES is also the best choice among cell culture buffers. Desheng Biochemical is a manufacturer specializing in the production of HEPES. Since its establishment in 2005, it has been Committed to the research of biological buffers, just to produce high-quality products, if necessary, please feel free to go to our official website for consultation! We look forward to hearing from you.