Desheng's six acridinium ester series have always been the company's flagship products. The feedback from the market has always maintained a high degree of recognition. This is inseparable from the quality problem of the product itself, and Desheng will help customers choose the appropriate acridinium ester series based on the information provided by the customer. Let's take a look at the different uses of different acridinium esters:
1. Acridine ester (DMAE-NHS): Chemiluminescence and immunoassay, receptor analysis, nucleic acid and peptide detection, etc.
2. Acridine ester (Me-DMAE-NHS): can be used for labeling proteins, antigens, antibodies, nucleic acids (DNA RNA), etc.
3. Acridine ester (NSP-DMAE-NHS): chemiluminescence and immunoassay, receptor analysis, nucleic acid and peptide detection
4. Acridinic acid propane sulfonate (NSP-SA): can be used for labeling proteins, antigens, antibodies, nucleic acids (DNA RNA), etc.
5. Acridine salt (NSP-SA-NHS): a very popular chemiluminescent label, whose stability, activity and sensitivity surpass those of radioactive isotopes
6. Acridine hydrazide (NSP-SA-ADH): The end of acridine hydrazide is suitable for reacting with polysaccharides, nucleic acids or proteins containing aldehyde groups to label them with acridine.
Advantages of acridine ester luminescent markers:
Large steric hindrance, high luminous efficiency and high luminous intensity
The luminescence reaction has few interference factors, and the label is stable (can be stored for several months at 2-8 ℃)
High thermal stability and high signal-to-noise ratio
Mild marking conditions
High marking rate
Does not affect separation after marking
Matters needing attention when using:
Take out the stock acridinium esters before weighing, adjust the advance time according to the state of the acridinium esters in the stock bottle, because it is frozen and stored, immediately opening the lid and weighing will cause the water vapor to condense due to the temperature difference, making the powder sticky and inconvenient to weigh quantity.
Acridinium ester is a chemiluminescent reagent, which is obvious to light, so in addition to freezing and avoiding light during storage, it should be frozen and protected from light as much as possible when shipping, especially if the weather is hot or long-distance transportation outside the country needs to pay more attention and take corresponding measures. Adjustment.
When in use, you can directly peel and weigh the acridinium ester in a container that dissolves it, and then add anhydrous DMF to dissolve it to avoid weighing loss or loss in the transfer process (in the past, some customers thought that insufficient acridine esters were received. ).