Agarose gel electrophoresis is an electrophoresis method using agarose as a medium, which has the dual functions of "molecular sieve" and "electrophoresis".

Agarose is a linear polysaccharide polymer extracted from red seaweed product agar. When the agarose solution is heated to the boiling point and cooled and solidified, it will form a good electrophoresis medium. Its density is determined by agarose. Determined by concentration.

The chemically modified low-melting agarose is relatively fragile in structure, so it will melt at a lower temperature and can be used for electrophoresis detection of nucleic acid fragments.

Agarose gel electrophoresis mainly detects the integrity and size of nucleic acid. As long as the nucleic acid is not too small or too large (beyond the separation range of electrophoresis), the reliability of this method is very high.

Nucleic acids have different charges depending on the pH, and the force in the electric field is different, so the running speed is different. Agarose gel electrophoresis is based on this principle to distinguish nucleic acids.

Nucleic acid molecules are negatively charged in a solution whose pH is higher than their isoelectric point and move toward the positive electrode in an electric field. Since the pentose-phosphate backbone of nucleic acids is structurally repetitive, nucleic acid chains of the same length have almost the same amount of net charge, so they can move toward the positive electrode at the same rate.

In addition, the gel containing agarose has a network structure, and the substance molecules will receive resistance when passing through, and the resistance of macromolecular substances will be large when they are surging. Therefore, in agarose gel electrophoresis, the separation of charged particles depends not only on the net The nature and quantity of the charge, but also depends on the molecular size, which greatly improves the resolution ability.

Under the same electric field strength, the migration speed of nucleic acid molecules depends on the size and configuration of the nucleic acid molecules themselves and the concentration of the agarose gel. In short, nucleic acids with larger molecular weights migrate slowly, and nucleic acids with smaller molecular weights The migration speed is faster, which is the basic principle of the application of gel electrophoresis technology to separate nucleic acid fragments.

Features and advantages of Desheng products:

You no longer need to purchase reagents such as agarose, nucleic acid dyes, electrophoresis solution, and loading buffer everywhere, all in one kit.

This kit can save you about an hour of experimental time.

a. It saves you the cumbersome glue making, and the precast glue is pre-stained with nucleic acid dyes, no electrophoresis solution dyeing or post-staining is required. Simple and convenient, ready to use.

b. This kit is specially equipped with high-pressure fast electrophoresis solution. If your nucleic acid sample fragment is below 2000bp, you can control the speed of electrophoresis at any time and adjust the voltage. The general mini gel can be completed in 5-10 minutes at the fastest; if The marker or nucleic acid sample you use in the experiment has many bands and long fragments (nucleic acid sample fragments greater than 2000bp), you need to adjust to a suitable low pressure according to the actual situation, or still use your original low-pressure electrophoresis conditions.

3. Use the DNA fragments obtained by electrophoresis of this product for gel recovery, which will not affect subsequent reactions such as DNA ligation.

Transportation and storage: Store and transport at 4°C. The validity period is 3 months. Do not store it below 0°C or above normal temperature. The precast glue in the kit will freeze crack or deform, which will affect your use.