Lysis refers to a state of cell division or rupture. Lysis buffer is a solution that helps to open cells. It can be used when some experimental analysis needs to extract cell contents. For example, scientists are extracting DNA from cells or Lysis buffer must be used to assist in protein analysis. There are many types of cell lysis buffers, among which the most common one is TRIS-HCL buffer.
TRIS-HCL can maintain the optimal pH value of the cell lysis buffer, so it is often used as the main component of the lysis buffer. The lysis buffer helps to open the cells and can screen for substances in the cells, such as salts, detergents, chelating agents and inhibitors, as well as some alkaline chemicals. It can not only distinguish and screen but also remove these substances.
In addition to the biological buffer, the lysate also includes a chelating agent and inhibitor, such as ethylenediaminetetraacetic acid (EDTA), because this chemical substance interacts with two positively charged metal ions (such as magnesium and calcium). Combining them can make them unusable for other reactions. Many DNases (proteins that chew DNA) and proteases (proteins that chop up other proteins) require magnesium ions to function, so by depriving these key components, EDTA helps reduce the level of protease or DNase activity.
Desheng is a manufacturer specializing in the production of biological buffers and EDTA. The biological buffer products currently produced include TRIS, TRIS-HCL, HEPES, TAPS, MOPS, CAPS, BICINE, EPPS, PEP, etc. A series of biological buffers, currently established For more than ten years, we have very rich R&D and production experience and product knowledge, which can provide customers with a large amount of technical support and after-sales guarantee. Please feel free to contact us if necessary!