Nucleic acid detection (real-time fluorescent RT-PCR method) is the gold standard for diagnosing new coronary pneumonia. In the specific process of nucleic acid detection, nucleic acid extraction is a very critical link. According to published reports, reagents such as TRIS, guanidine hydrochloride and EDTA play an important role in nucleic acid extraction.
Nucleic acid is a biological macromolecular compound formed by the polymerization of many nucleotides, including deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), and is one of the most basic substances of life. Nucleic acid extraction refers to the process of separating nucleic acids from samples using physical and chemical methods. It is the prerequisite technology for a series of molecular biological analysis such as nucleic acid amplification, DNA fragment connection, vector construction, and high-throughput sequencing. It is a life science It is a very important technology in research, biological timely application and genetic diagnosis. Therefore, the extraction of nucleic acid is of great significance.
Generally speaking, nucleic acid extraction is a multi-step work. First, biological sample materials such as cells and tissue materials need to be crushed to inactivate nucleases and release nucleic acids. The effects of TRIS, guanidine hydrochloride, EDTA and other reagents are mainly reflected in Release the nucleic acid link.
Nucleic acids (DNA, RNA) are easily hydrolyzed in acidic solutions, and are more stable in neutral or weakly alkaline solutions. And TRIS is Tris, pH buffer range: 7.0-9.0, can maintain the stability of the nucleic acid released after the lysis of the sample to be extracted, to avoid the degradation of the nucleic acid, and to improve the concentration and purity of the nucleic acid.
Guanidine hydrochloride is a strong inhibitor of nucleases, which facilitates the extraction of intact RNA from tissues rich in RNASE. Guanidine hydrochloride can not only destroy cell membranes quickly, but also denature proteins, so that proteins can be denatured and precipitated, so that nucleic acids can get rid of the entanglement of proteins.
EDTA is ethylenediaminetetraacetic acid, which can combine with metal ions such as Mg2+, Ca2+, Mn2+, Fe2+, and can prevent metal ions from activating proteases, thereby reducing the impact of metal ions on nucleic acid quality.
In summary, TRIS, guanidine hydrochloride, EDTA and other reagents can effectively lyse cells in the nucleic acid extraction process, so that the nucleic acid in the cell can be fully released, and a higher concentration of nucleic acid can be obtained, which is beneficial to improve the quality of nucleic acid and ensure subsequent operations. Accuracy. Recently, the demand for nucleic acid testing has increased, and the demand for TRIS has also increased. As a professional buffer manufacturer, Desheng can provide a large number of TRIS products for nucleic acid testing, which can meet the technical requirements of major manufacturers. If you need it, you can contact and order. Large quantity and favorable price!