Scientific research luminescent reagent acridine ester nsp series

 

In modern biomedical research, fluorescence labeling has become an indispensable technology, enabling researchers to monitor biomolecules in real-time, track cells, recognize proteins, study cell signal transduction, and conduct molecular diagnosis. The key to achieving these goals is fluorescent labeling reagents, among which acridine ester, as one of the luminescent labeling reagents, often appears in these studies. There are various types of them, including four types in the nsp series alone, including acridine salt NSP-SA, acridine salt NSP-SA-NHS, acridine hydrazide NSP-SA-ADH, and acridine ester NSP-DMAE-NHS. Below, we will focus on introducing the acridine esters in this series.

 

1、 Introduction to acridine ester NSP series

The acridine ester NSP series is a group of compounds widely used in the fields of biological detection and fluorescence labeling, both of which have high sensitivity and specificity. The following is a brief introduction:

 

1. Acridine salt NSP-SA: Introducing sulfonamide groups, it has high stability and strong resistance to hydrolysis. It is stable in acidic solutions (pH<4.8) and can be stored at room temperature for 4 weeks with protein conjugates. Its photoquantum yield does not decrease, and the freeze-dried product can be stored for more than one year at -20 ° C. At the same time, changes were made in hydrophilicity by introducing an internal salt of propionic acid, which increased its solubility and made it water-soluble with biomarkers such as antibodies and nucleic acids, enabling luminescence detection to be carried out in water. However, due to its acridine carboxylic acid nature, condensation agents such as EDCI need to be added in order to couple with proteins containing amino groups.

 

2. Acridine salt NSP-SA-NHS: This is a key fluorescent labeling substance with large steric hindrance, good hydrolysis stability, and high signal-to-noise ratio properties. Its photoquantum yield is superior to acridine ester AE-NHS, and can be applied in direct chemiluminescence analysis and chemiluminescence immunoassay. At the same time, NSP-SA-NHS chemiluminescence does not require any catalyst or enhancer, and can emit light in a dilute alkaline solution with hydrogen peroxide, thereby reducing background luminescence, improving signal-to-noise ratio, and reducing interference factors. In addition, there are many advantages in other places, such as rapid and concentrated light release, fast luminescence efficiency, high luminescence intensity, stable markers, and can be stored for six months at 2-8 ℃.

 

3. NSP-SA-ADH: It is an internal salt of acridine sulfonamide. Due to the different bond orders between C-N and C-O bonds, the C-N bond is greater than the C-O bond, and acridine amide compounds have strong resistance to hydrolysis. Because it is acridine hydrazide and contains free amino groups, the end of acridine hydrazide is suitable for direct coupling of polysaccharides, nucleic acids, or proteins containing aldehyde groups.

 

4. Acridine ester NSP-DMAE-NHS: NSP-DMAE-NHS is an ideal immunoassay biomarker for luminescence. The luminescence intensity of the NSP-DMAE-NHS conjugate with antibody (Ab) is higher, and it exhibits flashing luminescence, reaching the ideal peak at 0.4 seconds. Compared to the AE-NHS type, the thermal stability is also significantly improved. For example, at room temperature, in PB buffer with pH 7.0, after two weeks, the luminescence activity only decreased by 3.6%.

 

Although there are many series of acridine esters, their luminescent precursors and mechanisms are consistent, and their luminescent properties are not significantly different. Generally, the selection is based on experimental requirements. As a manufacturer integrating research, development, production, and sales of acridine esters, Desheng can supply different series of acridine esters for customers to choose from. At the same time, to ensure the reliability of the products, it can provide relevant testing reports and sample tests. At present, we have established our own reputation in the market and welcome customers to conduct on-site inspections and purchase. If it is inconvenient, please feel free to call or click on the website for more information at any time!