Introduction to TES sodium salts
TES sodium salt is a commonly used zwitterionic buffer in the field of biochemistry, commonly known as 2- [(tri (hydroxymethyl) amino] -1-ethanesulfonic acid sodium. CAS number 70331-82-7 has a white powder appearance and produces a colorless and transparent solution when dissolved in water. TES sodium salts have a wide range of applications in biological research, but cannot be used for Folin protein determination.
1、 Characteristics of TES sodium salt
1. Buffer interval. The pH range of TES sodium salt is 6.8-8.2. At room temperature, the pKa value is 7.5, and it has good buffering ability in the pH range of 6.5-8.1. The change in pH value is less affected by temperature, so it has strong practicality.
2. High stability. TES sodium salt has high thermal stability, and even when conducting relevant experiments in high-temperature environments, it will not affect the buffering effect.
3. Biocompatibility. The pH value of TES sodium salt is relatively close to its neutral value, making it compatible with various biological reagents and suitable for various biological experiments.
2、 The Use of TES Sodium Salt
1. Cell culture: TES sodium salt is suitable as a buffer for cell culture medium, stabilizing the pH value of the medium within the range suitable for cell survival.
2. DNA/RNA electrophoresis: TES sodium salt is a commonly used buffer component in DNA/RNA electrophoresis experiments, and has good stability in electrophoresis experiments.
3. Protein purification: TES sodium salt is also commonly used as a buffer for protein purification, regulating and stabilizing the appropriate pH value during the protein purification process.
4. Enzyme reaction: TES sodium salt can be used as a buffer for enzyme reactions, allowing them to occur within an appropriate pH range.
3、 Precautions for using TES sodium salt
TES sodium salt powder needs to be sealed during storage to avoid moisture and isolate air; All equipment and glassware used during the preparation process should be cleaned and disinfected; TES sodium salt solution needs to be prepared and used immediately. It is not recommended to pour the unused solution directly into the sewer, and preventive measures should be taken to avoid pollution.
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