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Hubei xindesheng Material Technology Co., Ltd

Hubei xindesheng Material Technology Co., Ltd

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Application and precautions of HEPES sodium salt

Release time: 2023-09-19

Application and precautions of HEPES sodium salt
HEPES sodium is a non volatile zwitterionic chemical buffer, fully known as N - (2-hydroxyethyl) piperazin-N '- (2-ethanesulfonic acid) sodium salt with CAS number 75277-39-3, used to regulate and stabilize the pH value of laboratory liquids. The appearance is white powder, and it usually needs to be prepared as a solvent when used, so it has good water solubility and relatively stable chemical properties.


Application of HEPES sodium salt
1. HEPES sodium salt has a low toxic effect on cultured cells, so it is commonly used as a buffer for cell culture research;
2. It can be used as a buffer in the phosphorylation detection process of permeable fibroblasts;
3. It can be used in combination with HEPES buffer and is a commonly used biological buffer formula;
4. HEPES sodium salt is also an effective inducer of lysosomal biogenesis;
5. It can also be used to form complex dialysis buffers for the determination of free thyroxine in serum through radioimmunoassay.


Precautions for the use of HEPES sodium salt
1. After the powdered HEPES sodium salt is prepared into a solution, it needs to be labeled in a timely manner to distinguish it from other solvents;
2. HEPES sodium salt needs to be stored in a sealed container, in a cool and dry place, and away from oxidants;
3. When impurities or mold appear in HEPES sodium salt powder or solution, it should be stopped and continued use;
4. Avoid direct contact with human skin. If accidentally touching the skin, immediately rinse with plenty of water. If you still feel unwell, seek medical attention as soon as possible;
5. HEPES sodium salts have a certain harmful effect on water, so unused HEPES sodium salt solutions need to undergo special treatment and meet relevant requirements before they can be discarded.


HEPES sodium salt has a wide range of uses and is also commonly used in the field of biological research. However, it is still necessary to carefully read the product manual when using it and meet the requirements when handling waste solvents. Hubei Xindesheng Material Technology Co., Ltd. specializes in the production of biological buffering agents. In addition to HEPES sodium salt, there are also a series of products such as TRIS and BICINE, and we can accept customized services. If you need more information, you can click on the official website or contact me!