What is the storage temperature for Bicine buffer?

 

Buffers are indispensable reagents in biochemical research, as they are used to maintain the stable pH of the solution and ensure that the experimental environment is not disturbed. Among them, Bicine (N, N-dihydroxyethylglycine) is a commonly used buffer, especially in enzyme reactions and electrophoresis. To ensure stability in these experiments, storage details should not be ignored, especially for powder and solution storage. It is recommended to operate according to regulations for effective use.

 

The effectiveness of Bicine buffer is influenced by storage conditions. Generally, the storage temperature is between 4 ° C and 25 ° C, so why do we need to store it within the range of 4 ° C to 25 ° C? There are three reasons for this:

 

1. PH stability: Bicine is an effective zwitterionic buffering agent that may cause its decomposition under high temperature conditions, thereby affecting its buffering performance. Therefore, storing it at a lower temperature, such as 4 ° C, helps to maintain its pH stability.

 

2. Humidity control: Bicine is a compound that is prone to moisture absorption. Absorbing humidity in the air can cause it to clump. If temperature control is not appropriate and indoor humidity increases, it can affect its shelf life.

 

3. Chemical stability: At higher temperatures, Bicine may undergo chemical reactions, leading to its decomposition and deterioration, which can be detrimental to experimental results. Therefore, storing it at an appropriate temperature helps to maintain its chemical stability.

 

Effective management of Bicine buffer:

1. Labeling and recording: Clearly mark the name, concentration, preparation date, and expiration date of Bicine on the storage container to track inventory and usage.

 

2. Sealed container: Ensure that the container containing Bicine is well sealed to prevent moisture and air pollutants from entering. Generally, sealed containers include dry bottles or sealed bags.

 

3. Regular inspection: Regularly inspect the appearance and performance of Bicine. If any abnormalities occur, such as clumping, color changes, odors, or measurement of pH deviation, replacement of raw materials should be considered.

 

4. Solution storage: If a solution needs to be prepared for storage, Bicine can be considered for refrigerated storage. In the refrigerated state, there will be no changes in a short period of time, but remember not to store it for a long time. Long term storage in the solution state can easily lead to bacterial growth.

 

In laboratory work, Bicine is an important buffer, so proper storage is necessary to ensure the accuracy of the experiment. By following appropriate storage temperature recommendations, the lifespan of Bicine buffer can be greatly extended and losses can be reduced. The Bicine produced by Desheng has high purity, and the powder raw materials are easy to store and transport, ready to use and reduce waste of raw materials caused by improper storage. At present, there is a large amount of stock available for sale in the company's warehouse. If you are interested, please feel free to contact us at any time!