Detailed Explanation of Trihydroxymethyl Aminomethane Knowledge

Trihydroxymethylaminomethane appears as a white crystalline powder with CAS number 77-86-1. At a temperature of 25 ℃, pKa is 8.1, and the pH value of the aqueous solution is between 10.5 and 12.0.

 

1. Advantages of Trihydroxymethyl Aminomethane

Trihydroxymethylaminomethane has strong alkalinity, wide buffering range, and strong practicality; It has little interference with chemical reactions in biological experiments and does not precipitate with calcium, magnesium, and heavy metal ions.

 

2. Application of Trihydroxymethyl Aminomethane

The application fields of trimethylaminomethane are very extensive. In addition to being used as a buffer in biological experiments and adjusting the pH value of experimental liquids, it can also be used in skincare products, new coatings, activated carbon, and pharmaceutical fields.

 

3. Common Buffer Systems of Trihydroxymethyl Aminomethane

① Trihydroxymethylaminomethane, combined with hydrochloric acid, sodium chloride, and potassium chloride, is used as a TBS buffer for cleaning immunostained tissues or Western blotting protein imprinting membranes;

 

② Trihydroxymethylaminomethane, combined with hydrochloric acid, sodium chloride, and tween20, is prepared as a TBST buffer, which is commonly used as a membrane washing buffer in protein immunoassay technology;

 

③ Trihydroxymethylaminomethane, combined with hydrochloric acid and ethylenediaminetetraacetic acid, is formulated as a TE buffer, which has a certain protective effect on the base of DNA and is therefore commonly used for the stability and storage of DNA;

 

④ The TAE buffer prepared by adding trimethylaminomethane with acetic acid and ethylenediaminetetraacetic acid is a widely used buffer system for short time electrophoresis of large DNA fragments;

 

⑤ Trihydroxymethylaminomethane, combined with boric acid and ethylenediaminetetraacetic acid, is used as a TBE buffer for long-term DNA electrophoresis, with good separation effect for smaller fragments.

 

4. Precautions for the use of trimethylaminomethane

The pH value of trimethylaminomethane solution is sensitive to changes in concentration and temperature, so when using it, attention should be paid to the influence of mixing ratio and temperature; In addition, attention should be paid to sealing, avoiding light, and moisture during storage, to prevent the influence of clumping and carbon dioxide.

 

5. Where to buy trimethylaminomethane

Hubei Xindesheng is a professional manufacturer of research and development and production of biological buffering agents, with rich experience and advanced technology. The produced trihydroxymethylaminomethane has high purity and good water solubility. Welcome to consult and purchase!