What buffer systems can TES buffer agent CAS7365-44-8 refer to?

TES buffer (CAS 7365-44-8), as a member of the Good's buffer series, is widely used in the fields of biochemistry and molecular biology. It is a zwitterionic buffer and also a part of the Tris buffer family. Due to its components including Tris, EDTA, and trace amounts of SDS, it is named TES buffer.

 

The pKa value of TES buffer at 25 ° C is exactly below the physiological pH value (7.4), which makes its effective pH range from 6.8 to 8.2. In addition, the dissociation constant of TES varies slightly with temperature, maintaining its stable buffering characteristics, which enables TES buffering agents to provide reliable buffering effects in many experiments.

 

However, it should be noted that in some fields, TES buffers may have different definitions. Sometimes, TES buffer does not only refer to trimethylolmethylaminoethanesulfonic acid, but also refers to other buffer systems. To avoid confusion, we need to be aware of it:

 

1. Tri (hydroxymethyl) methyl-2-aminoethanesulfonic acid: This compound has a structure very similar to trihydroxymethylaminomethane aminopropane sulfonic acid, with the difference being the absence of one methylene on the sulfonic acid main chain. Its CAS number is 7365-44-8, and its appearance is a white crystalline powder. It has a pH range of 3.5-5.0 in aqueous solution, an effective buffer range of 6.8-8.2, and a dissociation constant pKa (25 ° C) of 7.5.

 

2. Tris EDTA-SDS buffer: In some cases, TES buffer refers to a buffer system composed of Tris hydrochloric acid, EDTA (ethylenediaminetetraacetic acid), and trace amounts of SDS (sodium dodecylbenzenesulfonate), which is also referred to as TES buffer.

 

3. Tris EDTA NaCl buffer: In experiments such as plant protein extraction, the commonly used TE buffer (Tris+EDTA) may be added to a sodium chloride solution to form a TES buffer, which may appear in hemoglobin HB detection.

 

TES buffer, also known as Tris ethanesulfonic acid, meets various standards for Good's buffer like MES and BES, providing reliable support for biochemical experiments.

 

TES buffer is not only a zwitterionic ion buffer, but also refers to different buffer systems. The specific selection should be determined according to experimental requirements. Its unique buffering characteristics make it play an important role in protein analysis, enzyme activity measurement, and cell culture. As an advantageous supplier of TES buffer, Desheng can provide high-purity and diverse types of buffer raw materials, allowing customers to complete one-stop procurement, saving time and costs. If you have any interest, please feel free to contact us for purchase!