TES buffer is a commonly used biochemical buffer, often used in molecular biology, cell biology, and biochemical research. The synthesis process is relatively simple and easy to operate. Although it is usually easy to purchase this buffer on the market, if you need to use it in small amounts in experiments, you can also try making it yourself. Kind reminder, if you need to use it in large quantities to avoid errors, it is recommended to choose a professional manufacturer for procurement!

 

1、 Synthesis steps of TES

1. Three chemical substances need to be prepared, namely 2-amino-2-methyl-1,3-propanediol (AMPD), hydrochloric acid (HCl), and triethylene glycol (TEA);

2. Dissolve hydrochloric acid and AMPD in a reaction vessel, then gradually add TEA during the reaction process, stir and mix;

3. Dilute the solution to the desired concentration and adjust the TES solution to the desired pH value through a pH regulator.

 

2、 The application scope of TES

1. In molecular biology, TES buffer is often used as a buffer for experimental processes such as DNA and RNA conversion, electrophoresis, and PCR. Its unique buffering characteristics can stabilize the pH value of the experimental environment, thereby ensuring the accuracy and reliability of the experimental results.

 

2. TES buffer can also be used during cell culture to stabilize the pH value of the cell culture environment and provide appropriate ion balance, thereby maintaining normal cell growth and metabolism.

 

3. Another important application area is protein purification and analysis. The buffering ability of TES buffer can maintain the stability and solubility of proteins at specific pH values, providing favorable conditions for protein purification and analysis.

 

TES buffer has an important position and a wide range of applications in the field of biochemical research. By adjusting the pH value and providing a stable ionic environment, TES buffer can meet the requirements of researchers for buffer conditions in molecular biology, cell biology, and biochemistry experiments. Desheng is a manufacturer of TES buffer. Currently, the quality of TES produced is stable, with a purity of over 99%. If you have any purchasing needs, please feel free to contact us at any time. We look forward to your cooperation