What is the new trinder's reagent?

New trinder's reagents refer to highly water-soluble aniline derivatives, which are a large category of chromogenic substrates used in in vitro diagnostic kits to detect changes in liver function, kidney function, blood sugar, and blood lipids, thereby reflecting changes in the body. The principle of action of chromogenic substrates is generally to infer the content and activity of the measured substance by measuring the absorbance change of the chromogenic substrate.

The reaction principles of different chromogenic substrates also differ to some extent.

The trinder's reaction principle is to mix the new trinder's reagent 4-AAP with the corresponding oxidase and peroxidase to be tested. Due to the specificity of enzyme substances, they catalyze specific substances, and are sensitive to pH values, biological buffering agents need to be added to adjust to the appropriate pH range of enzyme substances. Add the substance to be tested to the above mixture for color reaction, producing a red quinone imine compound. Calculate the value of the tested item based on its absorbance.

Compared to traditional trinder's reagents, the new trinder's reagents have high water solubility and are more convenient to prepare and use; The UV absorption range of color reaction products is larger, so the reaction is more sensitive and can be determined for extremely small amounts of the substance to be tested. In addition, the new trinder's reagent reaction has relatively lenient requirements for acid-base reaction conditions and less restrictions on the use of enzymes, making it more practical in biochemical reactions.

At present, the common new trinder's reagents on the market include TOOS, TOPS, ADOS, ADPS, ALPS, DAOS, HDAOS, MADB, MAOS, etc. Different reagents have different advantages. For example, when detecting trace elements in serum, it is recommended to use TOOS, TOPS, and ALPS, which are more sensitive to color reactions; When precise detection is required, MADB and MAOS are recommended. These two reagents have longer absorption wavelengths and are less susceptible to interference from other substances.

Desheng has a history of 18 years in professional research and development of new trinder's reagents. The appearance of the produced new trinder's reagents is white crystal powder, with a purity of over 99%. The production process is stable, and the product batch differences are small. If you are interested, you can click on the official website to learn more details or contact me