Differences between Chemiluminescence Reagents Luminol and Acridine Esters

Chemiluminescence reagents refer to compounds that participate in energy transfer in luminescence reactions and ultimately release energy in the form of emitted photons. There are three common types of chemiluminescence reactions: enzymatic reaction luminescence, direct chemiluminescence, and electrochemical luminescence. The widely circulated and widely used chemiluminescent reagents on the market include Luminol reagent and acridine ester series reagent.

Luminol reagent belongs to the luminescent substrate of enzymatic reactions, with a pale white powder appearance, and is often used for detecting blood stains in criminal investigation. The oxidation reaction of luminol needs to be carried out in an alkaline liquid. Even without enzyme catalysis, it can slowly self emit light, but the luminescence sensitivity and duration will decrease.

Luminol reagent can only detect the presence or absence of blood stains, and cannot distinguish between human blood and animal blood. In addition, excreta can also react with Luminol to produce luminescence; However, the Lumino reaction does not damage the DNA in the bloodstain, so further testing can determine the source of the bloodstain. In addition, the use of hypochlorite bleach can interfere with the luminol luminescence reaction process.

Acridine ester series reagents
Acridine ester reagents belong to direct chemiluminescence reagents and do not require enzymatic catalysis. They can self emit light when the pH value of the solution is within an appropriate range, for example, in a dilute alkaline solution of hydrogen peroxide. Therefore, it is more convenient to use and is commonly used in research such as chemical immunoassay, receptor analysis, nucleic acid and peptide detection, as well as labeling of proteins, antigens, and antibodies.

There are six types of acridine ester series luminescent reagents produced by Desheng: DMAE-NHS, NSP-DMAE-NHS, NSP-SA, NSP-SA-NHS, NSP-SA-ADH, Me DMAE-NHS. The appearance of these reagents is yellow powder, with a purity of up to 98%. They have advantages such as high luminescence efficiency, high luminescence intensity, fast and concentrated light release, and few interference factors in the luminescence reaction.

Desheng has been researching chemiluminescence reagents for 18 years. In addition to luminol and acridine esters, there are also products such as isoluminol and luminol monosodium salts available for selection, as well as customized services. The production process is stable and the product batch differences are small. If you have a recent need to purchase chemiluminescent reagents, please click on the official website to learn more details!