Preparation of MES buffer for 2- (N-morpholine) ethanesulfonic acid monohydrate

2- (N-morpholine) ethanesulfonic acid monohydrate, abbreviated as MES, is a commonly used biochemical experiment buffer, mainly used to maintain the stability of biological systems within a specific pH range. The correct preparation method can ensure the accuracy and reliability of the experiment, and can help everyone efficiently complete the experimental operation. Below, we will specifically introduce the preparation method of 2- (N-morpholine) ethanesulfonic acid monohydrate MES buffer.

 

1、 Preparation method of MES buffer

1. Preparation of laboratory equipment: When preparing MES buffer, it is necessary to prepare a precision balance, burette, stirrer, distilled water, and some experimental containers to ensure the accuracy and hygiene of the experimental process.

 

2. Calculate required mass: Calculate the required mass of MES monohydrate based on the target concentration and volume. The following formula can be used to calculate mass: mass (g)=concentration (mol/L) ×  Volume (L) ×  Molecular weight (g/mol).

 

3. Add an appropriate amount of distilled water: In a clean container, add an appropriate amount of distilled water. It is usually recommended to use high-purity distilled water to avoid impurities affecting the experimental results.

 

4. Adding MES monohydrate: Slowly add the pre calculated MES monohydrate powder to distilled water. During the addition process, a magnetic stirrer can be used to thoroughly stir the solution to ensure full dissolution of MES.

 

5. PH adjustment: An important step in preparing MES buffer solution is to adjust its pH value. Use a pH meter to measure the initial pH value of the solution, and then adjust the pH value dropwise using hydrochloric acid or sodium hydroxide according to experimental requirements until the target pH value is reached. Be careful when adjusting the pH value to avoid excessive addition of acidic or alkaline solutions.

 

6. Dilute to target volume: After reaching the target pH value, dilute the solution with distilled water until the target volume is reached. During the dilution process, it is important to stir thoroughly to ensure a uniform solution.

 

7. Filtration and storage: After completion, a 0.22 micron filter membrane can be used to filter the solution and remove suspended small particles. Then store the prepared MES buffer in a sealed container to avoid contamination by dust and impurities.

 

2、 Preparation precautions

1. When preparing MES buffer solution, high-precision balance and accurate measuring tools should be used to ensure the accuracy of the ratio.

2. Be careful when adjusting the pH value, adding acid and alkali dropwise to avoid excessive adjustment.

3. Use experimental grade or molecular biology high-purity MES monohydrate to ensure the quality of the buffer solution.

4. The prepared MES buffer should be stored in a dry and dark environment to maintain its stability.

 

2- (N-morpholine) ethanesulfonic acid monohydrate MES buffer plays an important role in biochemical experiments. Through correct preparation methods, the quality and stability of the buffer can be ensured, thereby ensuring the accuracy and reliability of experimental results. As an advantageous supplier of MES, Desheng can provide high-purity raw material powders with a complete range of types for selection and personalized customization. If you are interested, please feel free to contact us!