Recommended Biological Buffers for Different Biochemical Experiments

Biological buffering agents are essential consumables in many biochemical experiments, and different buffer solutions may exhibit different effects under specific conditions. Therefore, it is necessary to choose appropriate buffer solutions based on specific experimental needs. Firstly, pay attention to the buffer range required in the experiment, and secondly, consider the changes in pH value and the concentration of the buffer solution during the experiment. This article will list suitable buffer solutions for different experimental needs.

 

1. Cell culture

In cell culture, buffer plays a crucial role in maintaining stable pH conditions in the culture medium. Common biological buffers such as HEPES and MOPS can effectively maintain the cell growth environment within the physiological pH range. In addition, TRIS buffer is often used for cell lysis and protein extraction.

 

2. Protein Research

Buffer is crucial for preserving the tertiary structure and activity of proteins. TRIS buffer is often used in electrophoresis experiments, while HEPES buffer has become a commonly used reagent for protein crystallization experiments due to its excellent buffering ability under neutral conditions.

 

3. Enzymatic reaction

Some buffers can regulate the rate and specificity of enzymatic reactions. For example, TRIS buffer is used for enzyme activity determination under acidic and neutral conditions, while HEPES buffer is suitable for alkaline environments.

 

4. Research on bacteria and eukaryotic cells

For studies involving bacteria and eukaryotic cells, MOPS is typically used to maintain its pH value as it can maintain stability within the physiological pH range.

 

When conducting research, careful consideration of the characteristics and applications of different buffer solutions can ensure the smooth progress of the experiment. As a manufacturer of biological buffer solutions, Desheng not only provides commonly used TRIS, HEPES, MOPS, CAPS and other buffer solutions, but also some products can be customized with indicators to meet specific customer requirements. Feel free to contact us if you need to make a purchase!