1,3-Bis ((trihydroxymethyl) methylamino) propane (Bis Tris) buffer range
Buffer plays a crucial role in biochemical and molecular biology experiments, as it can stabilize the pH value of the cell environment, maintain enzyme activity, and protect the stability of biological macromolecules. Among numerous buffer solutions, 1,3-bis ((trihydroxymethyl) methylamino) propane (Bis Tris) is highly favored by researchers due to its unique properties. The pH range of Bis-Tris buffer and its application in scientific research will be analyzed below to help better understand its characteristics and advantages.

1、 PH range of Bis Tris buffer
Bis-Tris buffer solution is a binary buffer system with two pKa values of varying sizes, with a primary acid dissociation constant Pka1=6.8 and a secondary acid dissociation constant Pka2=9.0. At room temperature, the working pH range of Bis-Tris buffer solution is 6.3-9.5. Within this range, Bis-Tris buffer solution can effectively maintain the stability of the solution and has a good protective effect on the stability of most biological macromolecules.

2、 Advantages of Bis Tris buffer
(1) Excellent buffering ability: The pKa value of Bis-Tris buffer solution is close to the neutral pH value, therefore it has extremely strong buffering ability under neutral conditions. It can effectively resist the influence of external acidic and alkaline substances on the pH value of the solution, and maintain the stability of the solution.

(2) Low background signal: In experiments such as electrophoresis and immunoblotting, it exhibits the characteristic of low background signal, which is conducive to accurately detecting the signal of target proteins and improving the reliability of experimental results.

(3) Protection of protein stability: Bis Tris buffer has a good stability protection effect on many proteins, which can maintain the natural conformation and function of proteins.

3. The Application of Bis Tris Buffer
Bis-Tris buffer is widely used in various experiments in the fields of biochemistry and molecular biology, including:
(1) Protein electrophoresis: commonly used in SDS-PAGE and Native-PAGE electrophoresis experiments to separate proteins and detect their relative molecular weight.
(2) Immunoblotting: Bis-Tris buffer is used as a protein transport buffer for immunoblotting experiments to detect the expression level of target proteins.
(3) Cell culture: Bis-Tris buffer can be used for the preparation of cell culture medium to maintain a stable pH value during the cell culture process.
(4) Nucleic acid electrophoresis: suitable for DNA and RNA electrophoresis experiments to detect the length and concentration of target nucleic acids.

1,3-Bis ((trihydroxymethyl) methylamino) propane (Bis Tris) buffer is widely recognized for its excellent performance. If you want to purchase this type of product, you can choose Desheng, which can provide analytical grade buffer, suitable for various experiments, and supplied at a discounted factory price. Feel free to contact us anytime!