What is the composition and function of the acridine ester labeled antibody kit?

The acridine ester labeled antibody kit is a commonly used tool for immunological research and biological analysis, used to detect and quantitatively analyze the presence and expression levels of specific proteins. So what exactly does it consist of? What are its functions? Below, we will focus on introducing this aspect to everyone.

 

1、 Composition of Acridine Ester Labeled Antibody Kit

1. Acridine ester labeled antibody: The kit contains specific antibodies that have already been labeled with acridine ester for the detection of specific proteins. Acridine ester itself serves as a luminescent probe that can achieve high sensitivity detection of target proteins by binding to antibodies.

 

2. Labeling agent: The reagent kit also contains a labeling agent for activating acridine ester labeling. This labeling agent can undergo a chemical reaction with acridine ester, covalently connecting it to the antibody, forming a labeled antibody complex.

 

3. Sample pretreatment buffer: In order to improve the detection sensitivity and specificity of the sample, the reagent kit usually includes sample pretreatment buffer to remove potential interfering substances and reduce non-specific background signals.

 

4. Reaction buffer: The reaction buffer is a key component of the reagent kit, providing appropriate conditions to induce specific binding reactions between labeled antibodies and target proteins. The reaction buffer usually includes components such as buffer, auxiliary reagents, and stabilizers.

 

2、 Common Functions of Acridine Ester Labeled Antibody Kit

1. Protein quantification: The acridine ester labeled antibody kit can be used to quantitatively analyze the expression level of the target protein. By binding with labeled antibodies, the target protein can generate a fluorescence signal, which can be used for quantitative detection and comparison of the content of the target protein in different samples.

 

2. Protein detection: can be used to detect the presence or absence of specific proteins. By specifically binding to the target protein, acridine ester labeled antibodies can generate fluorescence signals to confirm the presence of the target protein.

 

3. Immunohistochemistry: It has important applications in immunohistochemistry experiments. By binding to specific antigens, acridine ester labeled antibodies can display the location and distribution of target proteins in tissue slices or cells, helping researchers observe and analyze protein expression in biological samples.

 

4. Other auxiliary tests: In addition to the above functions, the acridine ester labeled antibody kit can also include other auxiliary modules, such as positive control samples, blocking reagents, and detection plates, to provide more complete and convenient experimental operations.

As an advantageous manufacturer of luminescent reagents, Desheng has rich experience and technology in quality control, technological innovation, and customer service. At present, there are Luminol, acridine ester series and other chemiluminescence products. Those interested in it are welcome to contact us to buy!