Instructions for the use of trihydroxymethyl aminomethane hydrochloride CAS1185-53-1

 

Trihydroxymethylaminomethane hydrochloride is a commonly used buffer solution in experiments, mainly composed of Tris and hydrochloric acid, with a CAS of 1185-53-1. It appears as a white crystalline powder and is easily soluble in water. Because of its strong buffer capacity, it can adjust the pH value of the solution and stabilize the reaction system, so it is often used in biochemical experiments and analytical chemistry. Below is an introduction to its use.

 

1、 Application field

1. It is mainly used in biochemical experiments and analytical chemistry, such as protein electrophoresis, enzyme activity determination, nucleic acid electrophoresis and other experiments as a buffer. By adjusting the pH value, you can avoid the influence of the acidity and alkalinity of the reaction system on the experimental results.

 

2. Tris HCl can also be used to prepare other compounds, such as amino acids, nucleotides, etc. During the preparation process, it is necessary to choose the appropriate concentration and ratio of hydrochloric acid according to experimental requirements.

 

2、 Instructions for use

1. Storage and preservation: Tris HCl should be stored in a dry and cool place, avoiding direct sunlight and being placed in a high-temperature environment. It is recommended to use a sealed container device to prevent the invasion of moisture and impurities. At the same time, it is necessary to pay attention to its stability and avoid prolonged exposure to the air or contact with harmful substances.

 

2. Preparation of buffer: Tris HCl can be used to prepare various buffer solutions, as follows:

(1) Prepare 0.01M Tris HCl buffer solution: add appropriate Tris HCl into deionized water and adjust the pH to about 2.0.

(2) Preparation of buffer solutions with different pH values: The dosage and pH value of Tris HCl can be gradually adjusted according to experimental needs to prepare buffer solutions with different pH values. For example, to prepare a buffer solution with a pH of 8.0, 3M Tris HCl and 10mM NaOH can be mixed and stirred until the alkaline solution precipitates completely, and then deionized water can be added to adjust the pH to around 8.0.

 

3、 Precautions for use

(1) To prevent Tris HCl powder from coming into contact with the skin, it is necessary to wear corresponding protective equipment such as gloves, masks, etc. when using.

(2) Tris HCl has strong buffering capacity, but there are also certain limitations, which are not suitable for all experiments. When using it, it is necessary to choose the appropriate concentration and ratio of hydrochloric acid according to the experimental needs to avoid affecting the experimental results.

(3) During use, it is necessary to strictly control the dosage and proportion of Tris HCl to ensure the effectiveness and accuracy of the reaction.

As an advantageous supplier of biological buffer solutions, Desheng has precise control over various indicators of Tris-HCl. Finished products sold must be repeatedly sampled and tested to meet standards before leaving the factory. Currently, the company has cooperated with thousands of customers and has a high turnover rate. If you are interested, please click on the website to inquire and purchase!