How long is the storage time of EDTA potassium sample?

 

When we go to the hospital for blood routine and ammonia tests, we will find that the color of the vacuum blood collection cap used by medical staff for blood collection is purple. As is well known, different colored caps are used to distinguish the different types of additives contained in vacuum blood vessels, while the additives in purple cap vacuum blood vessels are EDTA salts (dipotassium, tripotassium, disodium). When conducting blood cell counting and classification tests, the International Committee for Hematology Standardization recommends the use of EDTA potassium as an anticoagulant.

 

As a blood anticoagulant, the principle of action of EDTA potassium is to chelate with calcium ions or other divalent ions in the blood, blocking these ions from exerting coagulation effects and preventing blood coagulation from both endogenous and exogenous aspects. EDTA potassium has a certain protective effect on blood cells, does not affect the size and count of white blood cells, nor does it affect the morphology of red blood cells. However, it has an impact on the detection of calcium, potassium, and sodium plasma and is not suitable for the detection of these items.

 

Due to the non irreversible anticoagulant effect of EDTA dipotassium, in order to ensure the effectiveness of EDTA dipotassium use and the accuracy of the final results, relevant tests need to be completed within 24 hours of obtaining blood samples. When EDTA potassium samples are counted for different blood cells, there are also significant differences in storage time at different temperatures: white blood cell counts can be stored for 48 hours at 4 ℃, and can only be stored for 24 hours at 23 ℃; Platelets can only be stored for 24 hours at 4 degrees Celsius.

 

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