What are the storage conditions for MOPS buffer

 

MOPS is an acidic GOOD's buffer with a white crystalline powder appearance, odorless, and good water solubility. The CAS number is 1132-61-2. At 25 ℃, the pka is 7.2, and the pH of the aqueous solution is between 2.5 and 4.0. According to buffering theory, the buffering range of MOPS is between pH 6.5 and 7.9. Commonly used for preparing RNA electrophoresis buffer and protein purification chromatography buffer, it is particularly suitable for regulating and stabilizing the pH value of mammalian cell culture medium. Due to its strong practicality, many companies purchase MOPS in large quantities. Below is an introduction to its preservation methods.

 

1. Preservation of MOPS powder

The preservation of MOPS powder first requires sealing and moisture-proof to prevent contamination by dust and impurities, as well as to prevent the powder from deliquescence, which can affect the effectiveness of MOPS use. Secondly, MOPS is not suitable for high-temperature and high-pressure sterilization preservation, as the high-temperature and high-pressure environment not only plays a sterilization role, but also inactivates some active ingredients, affecting their effectiveness. Therefore, at room temperature, a dry and dark environment is more suitable for the storage of MOPS. It should be noted that long-term storage may result in slight clumping of MOPS, which does not affect the effectiveness of use.

 

2. Preservation of MOPS solution

After preparing MOPS into a solution, the principle of ready to use should be followed. When it is not enough to use and needs to be stored, it is necessary to first ensure that the stored instrument is clean and pollution-free to prevent the occurrence of bacterial spoilage in the future; Secondly, it is necessary to seal and store it to prevent prolonged exposure to air from damaging the pH value of the solution; The final storage temperature is 2-8 degrees Celsius, which is more suitable. The storage time should not exceed 6 months. Filter membranes can be used for sterilization, but it is also not suitable for high-temperature and high-pressure sterilization. When the storage time is relatively long and there are turbidity, odors, and impurities in the solution, the solution should not be used anymore.

 

Desheng Company is located in Ezhou City, Hubei Province. It has developed and produced various types of biological buffering agents for nearly 20 years. It is well-known in the biological buffering agent market, with stable production processes and small batch differences. The purity and water solubility of biological buffering agents are high, and they are widely recognized by users. If you also need biological buffer, you can contact me