How is the blood anticoagulant heparin lithium CAS9045-22-1 made?

 

Heparin lithium, as a commonly used reagent in blood testing, has a CAS number of 9045-22-1 and a white powder appearance. It has strong anticoagulant activity and significant effects, and has unique clinical value. After leaving the human body, if blood does not undergo any treatment from coagulation to precipitation, it not only takes a long time but also is difficult to ensure the accuracy of the results. The use of heparin lithium easily solves this problem. Many people don't know how it was made? So, let's focus on taking everyone to understand.

 

At present, the production methods of heparin lithium mostly use crude heparin sodium as raw material, which is refined and obtained by ion exchange at low temperature. This not only simplifies the production steps but also reduces production costs. The specific production process method is as follows:

 

1. Dissolve: Dissolve the weighed crude heparin in deionized water, and slowly stir evenly at room temperature;

2. Processing trypsin: After stirring and dissolving, start heating and adding ammonia water. During the reaction, accurately control the pH value, wash the precipitate with alcohol, and then dry it under vacuum conditions for later use;

3. Enzymatic hydrolysis: Add trypsin to the reaction solution, continue to heat up the enzymatic hydrolysis, filter to obtain green leaves, weigh the filtrate, collect trypsin, and adjust the pH value.

4. Oxidation: Then add hydrogen peroxide to the obtained filtrate and continuously adjust the pH value; Add sodium chloride and stir thoroughly to dissolve.

5. Ion exchange: Use a resin column to exchange ions in the obtained filtrate, then collect the effluent, add lithium salt, stir thoroughly to dissolve, and concentrate on the experimental filter membrane to collect the concentrated solution.

6. Precipitation drying: After precipitating the collected concentrated solution with ethanol, remove the clear liquid covering the upper layer, wash repeatedly until it becomes hard particles, then vacuum dry and crush to obtain high-quality heparin lithium.

 

The heparin lithium obtained in this way not only has high purity, but also stable performance, simple steps and low cost, making it a production method that most manufacturers are willing to use. Heparin lithium has good quality and ideal anticoagulant effect in clinical practice, so everyone must choose a supplier with reliable quality. As a professional manufacturer of heparin lithium, Desheng not only has decades of experience, but also has a product purity of over 99%, making it the first choice for most people. If you are interested, please click on the website to inquire about details and purchase!