What is PBS buffer? Look at the ratio and function here

 

In biochemical research experiments, PBS buffer is commonly referred to as an acid-base stabilizer. In addition to its buffering effect, it can also play a role in other industrial fields and medicine. At the same time, it can also be used to prepare different salt solutions, such as strong acid weak alkaline or weak acid strong alkaline, so that the stability of the experimental solution can be maintained by releasing its own acid and alkali during the reaction or preservation process. Below, we will introduce it specifically to you.

 

1、 What is PBS buffer solution?

Usually referred to as phosphate buffer salt, it serves as a solvent and can protect reagents after dissolution. It is also a common buffer system in biochemical experiments. Its main components include disodium hydrogen phosphate, potassium dihydrogen phosphate, sodium chloride, and potassium chloride. Due to the secondary dissociation of disodium hydrogen phosphate and potassium dihydrogen phosphate, the buffer pH range is wide, while sodium chloride and potassium chloride increase the concentration of salt ions.

 

2、 Ratio of PBS buffer solution

Taking the formulation containing 0.05% Tween-20 as an example:

1. Weigh an appropriate amount of potassium dihydrogen phosphate, disodium hydrogen phosphate, sodium chloride, and potassium chloride, and then add about 800 milliliters of deionized water to thoroughly stir and dissolve.

 

2. Then add concentrated hydrochloric acid to adjust the pH value, adjust it to a suitable range, and then adjust to a constant volume.

 

3. If high-temperature sterilization is required, it should be stored in a 4-degree refrigerator for future use after the operation is completed.

 

4. However, it is also important to pay attention to different types of experiments, and their ratios may vary. For example, 1x PBS and 2x PBS are solutions of different concentrations, which should be treated differently when used.

 

3、 The Role of PBS Buffer Solution

Based on its own equilibrium performance, it can be used to dilute active biological agents, as solutions such as distilled water and physiological saline may cause damage to active substances or cannot guarantee participation in the reaction in a suitable environment. Choosing to use PBS has become a rational choice. However, it should also be noted that PBS is not a versatile solution. Sometimes, depending on the increase of active substance conditions, such as the need to add other components to the equilibrium solution, PBS may not be suitable for use, and it is usually flexibly adjusted according to requirements.

 

As an advantageous supplier of biological buffer, Desheng has a complete range of buffer types, including several 10 types, for customers to inspect and choose from. At the same time, to ensure that customers save time, sample testing can be provided, and after passing the testing, bulk orders can be placed to avoid back-and-forth due to quality issues of the goods. If you are interested, please click on the website to inquire about details and purchase!