Acridine Ester Luminescence Analysis Study on DNA Damage of Formaldehyde and Acetaldehyde
Acridine ester can be used as a chemiluminescent indicator with a good intercalation structure of DNA double helix. Because low concentration of formaldehyde causes DNA breakage and damage, high concentration of formaldehyde causes DNA strand cross-linking, and acetaldehyde only causes DNA strand breakage damage. The chemiluminescence detection method for DNA break damage has the advantages of simplicity and sensitivity, and provides a simple research method for the study of environmental pollutant damage to DNA.
Acridinium-labeled streptavidin
Acridine ester DMAE·NHS was used to label streptavidin, one strain of TSH monoclonal antibody was labeled with biotin, and the other strain of TSH monoclonal antibody was coated on 96 micro-well plates, and the TSHBAS-CLIA method was established. Acridinium ester-labeled antibody or antigen, biotin-labeled antibody or antigen, streptavidin-coated magnetic beads with carboxyl or Tosyl (p-tosyl) functional groups; the particle size of the magnetic beads is 0.1-5μm . Since biotin and streptavidin are combined in a ratio of 4:1, the acridinium ester-biotin-antigen-antibody complex is also combined with streptavidin in a 4-fold ratio, so that the luminescence signal is also enlarged. 4 times, the detection sensitivity is higher.
Acridine ester-labeled antibody for rapid determination of antibody level in tuberculosis patients
Using acridinium ester-labeled goat anti-human IgG antibodies, a method for the determination of IgG in human serum has been established. The labeling method has mild conditions, is simple and easy to implement, and its standard curve ranges from 7.5 to 125 ng IgG/ml. The results of the determination of antibody levels in the serum of tuberculosis patients are good.
Acridine ester-labeled nucleic acid probe method can quickly detect Escherichia coli
Use the nucleic acid probe method to quickly detect E. coli O157:H7. The method is to detect E. coli O157:H7 by using a specific DNA probe labeled with acridine ester. The nucleic acid probe method detects E. coli O157:H7 with high specificity and sensitivity. The minimum concentration of E. coli O157:H7 is about 106cfu/ml. The result of detection of E. coli O157:H7 is consistent with the national standard method; for O157 : H7 identification time is only 30 minutes, which is simple and quick. The conclusion is that the nucleic acid probe method can be used for the rapid detection of E. coli O157:H7.