Acridine esters (NSP-SA-NHS) and related compounds have proven to be very advantageous chemiluminescent labels, with stability, activity and sensitivity exceeding those of radioactive isotopes. Acridine esters can react with any protein containing amino groups. Proteins, peptides, antibodies, and nucleic acids can all be labeled with acridine. The labeled compound emits light rapidly under the excitation of basic hydrogen peroxide, and the labeled compound can be detected by collecting photons.
NSP-SA-NHS luminescence principle
The main purpose of NSP-SA-NHS: Chemiluminescence and immunoassay, receptor analysis, nucleic acid and peptide detection, etc. In the alkaline H2O2 solution, the acridine ester is attacked by hydrogen peroxide ions to generate a tension and unstable dioxyethane, which is further decomposed into CO2 and electronically excited acridinone, when the acridinone returns In the ground state, a photon with a maximum absorption wavelength of 430nm is emitted.
NSP-SA-NHS product features
1 Before the luminescence reaction forms the electronically excited state intermediate, the non-luminescent substituted part attached to the acridine ring is separated from the luminescent part, so its luminous efficiency is basically not affected by the structure of the substituent.
2 No catalyst or enhancer is needed, and it can emit light in a dilute alkaline solution with H2O2.
3 The luminescence type is flash type. After adding the excitation solution, the luminous intensity reaches the maximum at about 0.4s, and the half-life is about 0.9s.
NSP-SA-NHS product advantages
1 Low background luminescence and high signal-to-noise ratio
2 Luminous reaction, less interference factors
3 Photon release is quickly concentrated, with high luminous efficiency and high luminous intensity
4 Easy to couple with protein, photon yield will not decrease after coupling, increase sensitivity, 2-8℃ can be stored for several months
5 The solid phase separator is a very fine magnetic powder, which can not only increase the coating area and speed up the reaction, but also can be cleaned at the same time.
NSP-SA-NHS product stability
1 It is stable in acidic solution (pH<4.8), and the protein conjugate is stored at room temperature for 4 weeks, and its photon yield does not decrease. The freeze-dried product can be stored for more than one year at -20°C.
2 When pH>4.8, especially in alkaline solution, the acridine compound is partially hydrolyzed to reduce its stability, and the hydrolysis process is a dark reaction process without luminescence; the degree of hydrolysis increases with the increase of pH, and with the increase of temperature High and increase.
3 The stability of acridine amide is higher than the structure of acridine ester, and its hydrolysis resistance is stronger.
4 Acridine ring or phenol ring or benzenesulfonyl ring is connected with a methyl group and other electricity-donating groups. Due to the large steric hindrance, the thermal stability is increased; the electron withdrawing group is attached, which is conducive to the nucleophilic substitution reaction Decreased stability.
Desheng is a domestic manufacturer specializing in the production of acridinium esters. It has always been favored by customers for its reliable quality and sufficient inventory. Desheng has developed and produced a total of 6 acridinium ester products with different groups, including: NSP-DMAE-NHS, DMAE-NHS, NSP-SA, NSP-SA-NHS, ME-DMAE-NHS, NSP-SA- ADH, customer satisfaction is very high, welcome to come and buy.