How to quickly synthesize biological buffer ACES using low-cost raw materials

 

ACES is a biological buffer with the Chinese name of N - (2-ethylphthalamido) - 2-aminoethanesulfonic acid. It is generally available in two forms on the market. The prepared solution or powder form can be selected according to your own needs. It has extremely special physical and chemical properties, so it can be used in many biochemical research experiments. Today's editor will teach you how to quickly synthesize the buffer ACES using low-cost raw materials.

 

The synthesis methods of ACES can also be found on the search engines of various platforms, but most of them are cumbersome and not applicable. The method described below is simple to synthesize and has low raw material prices. You can try it yourself at home.

Synthetic raw materials: chemically pure chloroacetamide, taurine, 95% ethanol;

 

Experimental equipment: three-necked flask, spherical condensing tube, cylindrical funnel, suction filter bottle, Buchner funnel, agitator, vacuum dryer, qualitative filter paper (9cm), CDR-1 differential thermal analyzer, PE580-B infrared spectrum analyzer;

 

Synthesis steps:

1. Quantitatively refluxing an aqueous solution mixture of chloroacetamide and taurine;

2. Adjust the reaction liquid to a certain pH value for reaction;

3. Adjust the reaction liquid to acidity after cooling it;

4. Obtaining a white crystal in an alcohol solution;

5. Drying in a vacuum dryer to obtain the finished ACES powder. (As mentioned above, ACES usually exists on the market in the form of powder or solution. The powder is used to facilitate storage and transportation. In practical applications, it needs to be configured as a solvent before it can be used.)

 

It is worth noting that the above only introduces a synthesis method of ACES, which cannot guarantee the quality of the finished product, and conclusions need to be drawn based on the specific operation process. If conducting important experiments, it is necessary to find formal ways to purchase products. Desheng is a manufacturer of ACES buffers. Currently, the ACES products produced are of high purity, meet various parameters, and have a favorable price. If you need to purchase, please come to consult and order!