Precautions for mops buffer solution

 

In biological experiments, biological buffers are often used to regulate and stabilize the pH value of experimental liquids, and mops is one of them. It belongs to GOOD's buffer, and has excellent water solubility. The buffer range is between 6.5 and 7.9. It is commonly used in in vitro diagnostic kits, protein nucleic acid isolation and purification, cell culture media, and biosynthesis and other related fields, with a wide range of applications.

 

To prepare 10xMOPS buffer solution, 41.8g of mops powder is required to be dissolved in about 700ml of DEPC treated water; Adjust the pH value to 7.0 using NaOH; Add 20 mL of 1 M sodium peroxyacetate and 0.5 M ethylenediaminetetraacetic acid (pH 8.0) treated with DEPC to the solution; Finally, fix the volume to 1L and use 0.45 μ M filter membrane filtration to remove impurities.

 

When preparing mops solutions, it is necessary to take care not to directly contact human skin with high-purity reagents. When using mops solutions, protective measures should be taken, relevant instruments to be used must be cleaned in place, and measuring tools used must also be calibrated in advance. When storing the prepared mops solution at room temperature, it is necessary to pay attention to sealing and avoiding light. "When exposed to light, mops solutions are prone to yellowing, and light yellow liquids do not affect their use, but if the color is too dark, they are not suitable for continued use.".

 

In addition, mops are not suitable for high-pressure sterilization. According to relevant research, when using high-pressure sterilization for mops, unknown yellowish substances can be generated as degradation products. Therefore, it is recommended to use membrane filtration with a pore diameter of 0.2 micrometers or less for mops sterilization. As mops is a highly purified chemical raw material, if the dosage used each time is very small, it is recommended to carry out subpackage treatment to facilitate subsequent use.

 

Hubei Xindesheng Material Technology Co., Ltd. is a professional manufacturer of various in vitro diagnostic reagents. It has been producing buffer reagents such as mops for more than ten years. The production process is stable, the product quality is better, the product purity can be above 99%, and the appearance can be completely white powder. Desheng's products have been sold to many regions at home and abroad, and are highly recognized by users. If you have any purchasing intentions, please contact me