The luminescent reagent acridine ester can accurately determine acetaldehyde, and the result can be obtained within 3 seconds at most!

 

With the development of the field of in vitro diagnosis, many experiments use luminescent reagents to complete the detection, among which the more popular reagent is acridine ester, which can use its own characteristics to achieve the purpose of rapid labeling substances. In addition, due to its advantages of mild reaction conditions, very sensitive detection, rapid luminous efficiency, and high luminous intensity, it will also be widely used in biochemical reagent kits, environmental monitoring, and pharmaceutical analysis. Due to the recent attention paid to environmental issues by various places, many people will use acridine ester as an auxiliary test, so the following will focus on this aspect to introduce to everyone.

 

In fact, the role of acridine ester in environmental monitoring is to measure the content of acetaldehyde in environmental water samples, which can help everyone get timely improvement treatment in order to prevent unnecessary trouble in the future.

 

1、 Determination of acetaldehyde content with acridine ester

Acetaldehyde is generally stored in rivers, lakes, and the atmosphere in the surrounding environment. Although its content is not high, it can cause significant environmental pollution, especially for humans, and can pose a threat to life safety. Currently, spectrophotometry, gas chromatography, and liquid chromatography are commonly used for the determination of acetaldehyde content, but most of them lack sensitivity and cannot respond in a timely manner. Therefore, it was proposed that an analytical method coupled with enzyme reaction and acridine ester chemiluminescence reaction could be used for detection. Upon unexpected discovery, the reaction was rapid and the results could be quickly seen in a short period of time, so it began to be popularized.

 

2、 Detection principle of acridine ester

The principle of its action is that when exposed to acridine ester, it will be oxidized to acetic acid and H2O2 under the action of purinase. The H2O2 generated by the enzyme reaction can react with acridine ester, and the luminescence is flash type. The reaction can be completed quickly within 3 seconds. If the luminescence intensity is within a certain range of requirements, the amount of its content can be detected.

 

3、 Inspection precautions

It is important to note that during the entire detection process, the selection of acridine ester must be of analytical purity, and the pH value should be maintained at around 7.5, otherwise there will be errors in the detection effect.

 

As a professional research and development manufacturer of chemiluminescence reagents, Desheng can provide a series of products such as acridine esters and luminol, all of which are of analytical purity level, with a purity of up to 99%, fully meeting customer experimental requirements. If you have any interest, please click on the website to inquire about the details and purchase!