Effect of EDTA dipotassium on platelet and sodium detection

 

The International Committee for Standardization of Hematology recommended potassium EDTA as the first choice of anticoagulant in the relevant test items of blood cell count and classification, because it has the advantages of little influence on the morphology of red blood cells and white blood cells, and can inhibit the aggregation of platelets in vitro, but it also has some effects in normal use.

 

1. Effect on platelets

When used as an anticoagulant, potassium EDTA can cause abnormal adhesion and aggregation of platelets. However, the blood cell counter counts platelets according to their volume and the number of pulses generated when they pass through micropores. When platelets aggregate into larger particles, the instrument only recognizes the size of particles and cannot distinguish the nature of particles, resulting in multiple platelets being counted as a single count, or being mistaken for small red blood cells and not included in the count range of platelets, Reduce the platelet count, resulting in deviation of test results.

 

If the platelet count is lower than the normal value, the sodium citrate anticoagulant can be used for platelet test, or the anticoagulant suitable for platelet count can be used for retest.

 

2. Effect on sodium detection

The coagulation mechanism of EDTA dipotassium is to chelate with calcium ions or other bivalent ions in blood samples to prevent it from acting as a cofactor of thrombin, thus preventing blood coagulation. Therefore, it is not suitable for the detection of sodium ions, nor for the determination of calcium ions, potassium ions, iron ions and other elements, as well as PCR experiments. In case of items requiring sodium ion detection, the use of EDTA potassium should be avoided.

 

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